Annals of Clinical Microbiology, The official Journal of the Korean Society of Clinical Microbiology

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pISSN 2288-0585 eISSN 2288-6850

Selection of Unnecessary Urine Culture Specimens Using Sysmex UF-5000 Urine Flow Cytometer

Original article

Annals of Clinical Microbiology (Ann Clin Microbiol) 2018 December, Volume 21, Issue 4, pages 75-79.

https://doi.org/10.5145/ACM.2018.21.4.75

Selection of Unnecessary Urine Culture Specimens Using Sysmex UF-5000 Urine Flow Cytometer

Duyeal Song, Hyun-Ji Lee, Su Yeon Jo, Sun Min Lee, Chulhun L. Chang

Department of Laboratory Medicine, Pusan National University School of Medicine, Yangsan, Korea

Abstract

Background: Urine culture is one of the most frequently requested tests in microbiology. Automated urine analyzers yield much infection-related information. The Sysmex UF-5000 analyzer (Sysmex, Japan) is a new flow cytometry urine analyzer capable of quantifying urinary particles, including bacteria, WBCs, and yeast-like cells (YLCs) and can provide a Gram stainability flag. In this work, we evaluated how many unnecessary urine cultures could be screened out using the UF-5000.

Methods: We compared the culture results of 126 urine samples among 453 requested urine cultures (from sources other than the Urology and Nephrology departments) with urinalysis results. Urine cultures were considered positive if bacterial or YLC growth was ≥104 CFUs/mL.

Results: We used urinalysis cut-off values of 50/μL and 100/μL for bacteria and YLC, respectively. Forty eight of the 126 (38.1%, or 10.6% of 453 requested) cultures were below these cut-off values and did not contain any culture-positive samples.

Conclusion: Bacteria and YLC counts generated using the UF-5000 analyzer could be used to screen out negative cultures and reduce urine culture volume by ∼10% without sacrificing detection of positive cultures. (Ann Clin Microbiol 2018;21:75-79)

Keywords

Sysmex UF-5000 flow cytometer, Unnecessary urine cultures, Urinary tract infection