Annals of Clinical Microbiology, The official Journal of the Korean Society of Clinical Microbiology

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pISSN 2288-0585 eISSN 2288-6850

Antimicrobial Susceptibility and Genetic Analysis of Campylobacter jejuni Isolated from Diarrhea Patients in Busan

Original article

Annals of Clinical Microbiology (Ann Clin Microbiol) 2019 June, Volume 22, Issue 2, pages 42-49.

https://doi.org/10.5145/ACM.2019.22.2.42

Antimicrobial Susceptibility and Genetic Analysis of Campylobacter jejuni Isolated from Diarrhea Patients in Busan

Sun Hee Park, Byeong Jun Kim, Young Hee Kwon, In Yeong Hwang, Gyung Hye Sung, Eun Hee Park, Sung Hyun Jin

Microbiology Team, Department of Infectious Disease Research, Busan Metropolitan City Institute of Health & Environment, Busan, Korea

Abstract

Background: Campylobacter jejuni is an important food-borne pathogen that causes human gastroenteritis. This study was conducted to investigate the incidence of isolation, antimicrobial susceptibility pattern, and C. jejuni genotype from diarrhea patients in Busan, Korea.

Methods: A total of 97 C. jejuni were isolated from diarrhea patients during five food-borne outbreaks from 2014 to September 2017. Antimicrobial susceptibility tests were carried out by the broth microdilution method for ciprofloxacin (CIP), nalidixic acid (NAL), tetracycline (TET), chloramphenicol, azithromycin (AZI), erythromycin (ERY), streptomycin (STR), gentamicin, and telithromycin. To investigate C. jejuni genotypes, pulsed-field gel electrophoresis (PFGE) profile analysis was performed.

Results: The isolation rate of C. jejuni was 2.0% for the last 4 years and increased annually. Antimicrobial resistance rates of C. jejuni were shown to be in the order of NAL (90.9%), CIP (89.4%), TET (13.6%), AZI (3.0%), ERY (3.0%), and STR (1.5%). The proportion of multidrug-resistance was 18.2%, and they commonly contained quinolones (CIP-NAL). Analysis of PFGE patterns of SmaI-restricted DNA of C. jejuni isolates showed 17 clusters; cluster 11 was the major genotype pattern.

Conclusion: This study will provide useful data for the proper use of antimicrobials and the management of resistant C. jejuni. Also it will help to provide data for the epidemiological investigation of food- borne diseases caused by C. jejuni, which is expected to increase in the future. (Ann Clin Microbiol 2019;22:42-49)

Keywords

Antimicrobial susceptibility, Campylobacter jejuni, Pulsed-field gel electrophoresis