Annals of Clinical Microbiology, The official Journal of the Korean Society of Clinical Microbiology

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pISSN 2288-0585 eISSN 2288-6850

Cellulitis Caused by a Novel Cupriavidus Species Strain J1218 Identified by Whole Genome Sequencing

Case report

Annals of Clinical Microbiology (Ann Clin Microbiol) 2019 December, Volume 22, Issue 4, pages 105-109.

https://doi.org/10.5145/ACM.2019.22.4.105

Cellulitis Caused by a Novel Cupriavidus Species Strain J1218 Identified by Whole Genome Sequencing

Young Jin Ko1,2, Soo-Young Yoon2, Chae Seung Lim2, Chang Kyu Lee2
1Department of Laboratory Medicine, Chosun University Hospital, Gwangju, 2Department of Laboratory Medicine, Korea University College of Medicine, Seoul, Korea

Abstract

We report a case of cellulitis caused by a novel Cupriavidus species identified using whole-genome sequence analysis. Subcutaneous tissue biopsies from the left lower leg of a 67-year-old man who suffered from cellulitis were cultured. Round, convex, gray and non-hemolytic colonies were recovered after 72-h incubation. 16S rRNA sequence analysis showed 98.6% similarity with Cupriavidus basilensis DSM 11853(T) in the NCBI database and 99.9% similarity with C. basilensis KF708 in the EzBioCloud database. Genomic analysis using the MiSeq platform (Illumina, USA) and the TrueBac ID database (ChunLab, Korea) revealed that the average nucleotide identity (ANI) of this strain with C. basilensis DSM 11853(T) was 87.6%. The patient was treated with oral cefditoren pivoxil for 9 weeks. This study is the first to report cellulitis caused by Cupriavidus species strain J1218. (Ann Clin Microbiol 2019;22:105-109)

Keywords

Cellulitis, Cupriavidus, Whole-genome sequencing, 16S rRNA