Original article PDF Hee Jin Huh1, Eu Suk Kim2, Seok Lae Chae1 1Department of Laboratory Medicine, Dongguk University Ilsan Hospital, Goyang, 2Department of Internal Medicine, Seoul National University Bundang Hospital, Seongnam, Korea Corresponding to Seok Lae Chae, E-mail: rocky@dumc.or.kr Ann Clin Microbiol 2011;14(2):74-78. https://doi.org/10.5145/KJCM.2011.14.2.74Copyright © Korean Society of Clinical Microbiology. Abstract Background: Methicillin-resistant Staphylococcus aureus (MRSA) is a major nasocomial pathogen. The active surveillance of MRSA is essential to limit its transmission. The BD GeneOhm MRSA real-time PCR assay (Becton Dickinson Diagnostics, San Diego, USA) has been recently developed and used for same-day MRSA detection directly from nasal swab specimens. The authors of the present study compared GeneOhm MRSA PCR with culture methods to evaluate its diagnostic performance for MRSA active surveillance.  Methods: The present study was conducted on patients admitted to the ICU for six months. A total of 371 nasal swab specimens were obtained from patients at admission

Review article Eun-Jung Cho1, Eun Jin Lee1, Younggil Cha2,3 Nuri Lee1, Ki Ho Hong4, Hee Jin Huh5, Young Joo Cha6, Hyun Soo Kim1 1Department of Laboratory Medicine, Hallym University College of Medicine, Chuncheon, 2Molecular Diagnostic Division, Bioneer Corp., Seoul, 3College of Pharmacy, Kangwon National University, Chuncheon, 4Department of Laboratory Medicine, Seoul Medical Center, Seoul, 5Department of Laboratory Medicine, Dongguk University College of Medicine, Goyang, 6Department of Laboratory Medicine, Chung-Ang University College of Medicine, Seoul, Korea Corresponding to Hyun Soo Kim, E-mail: hskim0901@empas.com Ann Clin Microbiol 2020;23(2):57-66. https://doi.org/10.5145/ACM.2020.23.2.2Received on 1 November 2019, Revised on 24 December 2019, Accepted on 24 December 2019, Published on 20 June 2020.Copyright © Korean Society of Clinical Microbiology. Abstract Molecular diagnostic techniques are used for the diagnosis and monitoring of viral infections. The performance of the in vitro diagnostic assays is important for an accurate and prompt diagnosis. Positive clinical samples or reference materials (RMs) are essential

Original article Aram Kim, Heerah Lee, Kyu-Hwa Hur, Heungsup Sung, Mi-Na Kim Department of Laboratory Medicine, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Korea Corresponding to Mi-Na Kim, E-mail: mnkim@amc.seoul.kr Ann Clin Microbiol 2020;23(4):191-199. https://doi.org/10.5145/ACM.2020.23.4.4Received on 23 August 2020, Revised on 28 Septenber 2020, Accepted on 13 October 2020, Published on 20 December 2020.Copyright © Korean Society of Clinical Microbiology. Abstract Background: Inconclusive SARS-CoV-2 real-time reverse transcription-PCR (rRT-PCR) test results, which are positive for one or more target genes but not all, are problematic in clinical laboratories. In this study, we aimed to investigate the cause and clinical relevance of such inconclusive results. Methods: rRT-PCR was performed using the Allplex 2019-nCoV assay kit (Seegene Inc., Korea) targeting the following three genes: E, RdRp, and N. For all inconclusive test results reported from March to June 2020, the frequency per kit, lot number, specimen type, cycle threshold (Ct)

Original article PDF Jin Hee Park, M.D., Jung Won Huh, M.D. and Mi Ae Lee, M.D. Department of Clinical Pathology, Ewha Womans University College of Medicine, Seoul, Korea Corresponding to Mi Ae Lee Ann Clin Microbiol 2000;3(1):48-52.Copyright © Korean Society of Clinical Microbiology. Abstract Background: The diagnosis of tuberculosis has been based on the detection of tubercle bacilli by acid-fast stain of smear or cultures, and recently the serologic diagnosis of tuberculosis has been provided a means of sensitive and specific detection of Mycobacterium tuberculosis. We evaluated the utility of enzyme immunoassay using determiner Tuberculosis Glicolipids (TBGL) antibody kit (Kyowa Medex Co. Ltd, Japan) to detect anti-TBGL antibody for diagnosis of pulmonary tuberculosis. Methods: Anti-TBGL antibody assay was performed to the sera from 44 patients with active pulmonary tuberculosis (17 patients with smear positive, 7 patients with only culture positive, 20 patients with clinically active tuberculosis) and 80 controls (30 healthy controls, 24 patients with non-tuberculous

Original article PDF Wee Gyo Lee1, Il Joong Park1, Ji Young Huh2, Eui-Chong Kim2, Kyungwon Lee3, Mi-Na Kim4, Chulhun L. Chang5, Sunjoo Kim6, Young Uh7, Insoo Rheem8, Gyoung Yim Ha9, Hye Soo Lee10 Department of Laboratory Medicine1, Ajou University School of Medicine, Suwon; Department of Laboratory Medicine2, Seoul National University College of Medicine; Department of Laboratory Medicine3, Yonsei University College of Medicine; Department of Laboratory Medicine4, University of Ulsan College of Medicine, and Asan Medical Center, Seoul; Department of Laboratory Medicine5, Pusan National Uinversity School of Medicine, Busan; Department of Laboratory Medicine6, Gyeongsang University College of Medicine, Jinju; Department of Laboratory Medicine7, Yonsei University Wonju College of Medicine, Wonju; Department of Laboratory Medicine8, Dankook University College of Medicine, Chunan; Department of Laboratory Medicine9, Dongguk University College of Medicine, Gyungju; and Department of Laboratory Medicine10, Chonbuk National University College of Medicine, Chonju, Korea Corresponding to Wee Gyo Lee, E-mail: weegyo@ajou.ac.kr Ann

Case report PDF Sae-Mi Lee, Young-Jin Kang, Hee Jae Huh, Chang-Seok Ki, Nam Yong Lee Department of Laboratory Medicine and Genetics, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Korea Corresponding to Hee Jae Huh, E-mail: heejae.huh@samsung.com Ann Clin Microbiol 2015;18(4):135-139. https://doi.org/10.5145/ACM.2015.18.4.135Copyright © Korean Society of Clinical Microbiology. Abstract Yokenella regensburgei, a member of the family Enterobacteriaceae, is rarely isolated in humans. Here, we report a 71-year-old man with diabetic foot infection from which Y. regensburgei was isolated. Following debridement and disarticulation of the foot, an exudate specimen was obtained, from which Gram- negative bacilli were recovered. The organism was identified as Y. regensburgei using the Vitek 2 system (bioMérieux, USA) and 16S rRNA and gyrB gene sequencing. To our knowledge, this is the first case of Y. regensburgei isolation in Korea. (Ann Clin Microbiol 2015;18:135-139) Keywords Diabetic foot, DNA sequencing, Yokenella regensburgei