Fig. 2. Phylogenomic placement of the clinical Segniliparus rugosus isolate by Type Strain Genome Server (TYGS). A genome-based tree was generated with the TYGS (accessed September 5, 2025) using Genome BLAST Distance Phylogeny (GBDP) distances and FastME. The isolate clustered within the S. rugosus clade together with the type strain, supporting the species assignment. The tree was visualized using iTOL v7. Node labels indicate GBDP pseudo-bootstrap support values (only ≥ 50% are shown), and the scale bar indicates GBDP distance.
Ann Clin Microbiol 2025;29(1):2. Pulmonary infection caused by Segniliparus rugosus in a patient without systemic immunodeficiency but with bronchiectasis: first identification by matrix-assisted laser desorption ionization time-of-flight mass spectrometry with whole-genome confirmation Download image
Fig. 1. Colony morphology and acid-fast staining of Segniliparus rugosus. (A) Colonies on Ogawa medium. Rough, dry, buff-to-orange colonies. (B) Acid-fast bacillus stain (colony smear). Strongly acid-fast short rods were observed, unlike Nocardia spp., which are typically weakly/ partially acid-fast and show branching filaments.
Ann Clin Microbiol 2025;29(1):2. Pulmonary infection caused by Segniliparus rugosus in a patient without systemic immunodeficiency but with bronchiectasis: first identification by matrix-assisted laser desorption ionization time-of-flight mass spectrometry with whole-genome confirmation Download image
Pulmonary infection caused by Segniliparus rugosus in a patient without systemic immunodeficiency but with bronchiectasis: first identification by matrix-assisted laser desorption ionization time-of-flight mass spectrometry with whole-genome confirmation
Letter to the editor Jaesoon Kim1,2*, Nakwon Kwak3,4*, Jee-Soo Lee1,2, Taek Soo Kim1,2, Moon-Woo Seong1,2, Jae Hyeon Park1 1Department of Laboratory Medicine, Seoul National University Hospital, Seoul, Korea2Department of Laboratory Medicine, Seoul National University College of Medicine, Seoul, Korea3Division of Pulmonary and Critical Care Medicine, Department of Internal Medicine, Seoul National University Hospital, Seoul, Korea4Department […]