Annals of Clinical Microbiology, The official Journal of the Korean Society of Clinical Microbiology

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Indexed in KCI, KoreaMed, Synapse, DOAJ
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pISSN 2288-0585 eISSN 2288-6850

Evaluation of a Rapid Immunochromatographic Assay Kit for the Diagnosis of Salmonella Typhi Infection

Original article

Annals of Clinical Microbiology (Ann Clin Microbiol) 2007 October Volume 10, Issue 2, pages 123-127.


https://doi.org/10.5145/ACM.2007.10.2.123

Evaluation of a Rapid Immunochromatographic Assay Kit for the Diagnosis of Salmonella Typhi Infection

Sun Min Lee, M.D., Young Jin Kim, M.D., Sang Hyun Hwang, M.D., Eun Yup Lee, M.D., Han Chul Son, M.D., Chulhun L. Chang, M.D.

Department of Laboratory Medicine, School of Medicine, Pusan National University, Busan, Korea

Abstract

Background: Widal test, whose sensitivity and specificity are variable from region to region depending on the incidence of typhoid fever, is still in use and shows a relatively low positive predictive value in Korea. We evaluated a rapid immunochromatographic assay (ICA) kit for the rapid diagnosis of Salmonella Typhi infection.

Methods: Twenty five patients with Salmonella Typhi bacteremia were selected for the patient group. For the control group, 21 nontyphoidal salmonellosis patients, 56 non-Salmonella bacteremia patients, and 57 healthy individuals were selected for evaluating the specificity of ICA test. Each serum specimen was tested with the SD Salmonella Typhi IgG/IgM kits (Standard Diagnostics, Inc., Yongin, Korea).

Results: The ICA showed a sensitivity of 100% in Salmonella Typhi patients, and specificities of 61.9%, 82.1% and 91.2% in nontyphoidal salmonellosis patients, non-Salmonella bacteremia patients, and healthy individuals, respectively. The ICA test showed a high sensitivity but a low specificity when compared with the Widal test.

Conclusion: The ICA test by SD Salmonella Typhi IgG/IgM kit is highly sensitive, but its specificity is relatively low. The ICA test is simple, objective, and suitable for screening typhoid fever. (Korean J Clin Microbiol 2007;10:123-127)

Keywords

Salmonella Typhi, Immunochromatographic assay, Typhoid fever, Widal test