Annals of Clinical Microbiology, The official Journal of the Korean Society of Clinical Microbiology

6

Weeks in Review

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Weeks to Publication
Indexed in KCI, KoreaMed, Synapse, DOAJ
Open Access, Peer Reviewed
pISSN 2288-0585 eISSN 2288-6850

Search Results for: Young Ree Kim

Misidentification as Vibrio alginolyticus with Vitek GNI+ Card in Three Cases

Case report PDF Gyoung Yim Ha, and Moon Yeun Kim* Department of Laboratory Medicine, College of Medicine, Dongguk University, Kyongju, Korea Corresponding to Moon Yeun Kim, E-mail: kmy@dongguk.ac.kr Ann Clin Microbiol 2005;8(1):99-104.Copyright © Korean Society of Clinical Microbiology. Abstract The introduction of a new, fully automated system into the clinical microbiology laboratory contributes to a rapid identification of microorganisms with accurate and reliable results, but such a system requires a high cost and additional tests for identification of some species. For instance, additional tests on oxidase, indole, motility, hemolysis, and pigmentation are needed in the correct identification by using Vitek GNI+ system (bioMerieux Vitek Inc., MO, USA). In particular, Vibrio and Aeromonas species are occasionally identified incorrectly when an automated system is used, and thus conventional biochemical tests may be more reliable in the identification of such species. We experienced three cases of incorrect identification of Vibrio parahaemolyticus, Vibrio cholerae, and Aeromonas veronii biovar sobria as Vibrio alginolyticus by using

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Detection of the Causative Agents of Traveler’s Diarrhea Using a Real-Time PCR Screening Method

Original article PDF Semi Jeon1, Junyoung Kim1, Harim Lee2, Minyoung Son2, Misun Park1, Bokkwon Lee1, Seonghan Kim1 1Division of Enteric Bacterial Infections, National Institute of Health, Seoul, 2Gimhae National Quarantine Station, Gimhae, Korea Corresponding to Seonghan Kim, E-mail: kkingsh@chol.com Ann Clin Microbiol 2009;12(4):186-192.Copyright © Korean Society of Clinical Microbiology. Abstract Background: The incidence of infectious diarrheal disease in Korea has decreased over the past decade, but traveler’s diarrhea (TD) is increasing in frequency. We therefore investigated the distribution of the causative agents of TD. Methods: A total of 132 rectal swab specimens were acquired from TD patients who entered the country via Gimhae International Airport. The specimens were screened for 12 bacterial pathogens by real-time PCR, and target pathogens were isolated from the PCR positive specimens using conventional microbiological isolation methods. Results: A total of 93 specimens (70.5%) showed positive PCR screening results, and of these specimens, nine species and 50

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Evaluation of OraQuick Advance Rapid HIV-1/2 Antibody Test as a Screening Test for HIV Infection

Original article PDF Tae Youn Choi1, Young Ik Seo1, Tae Hyong Kim2, Jeong Won Shin1, Rojin Park1 Departments of 1Laboratory Medicine and 2Internal Medicine, Soonchunhyang University Hospital, Seoul, Korea Corresponding to Tae Youn Choi, E-mail: choity@hosp.sch.ac.kr Ann Clin Microbiol 2009;12(3):116-121.Copyright © Korean Society of Clinical Microbiology. Abstract Background: For the diagnosis of HIV infection, enzyme immunoassay (EIA) or chemiluminescence immunoassay (CLIA) is commonly used as a screening test. Although these methods have a high sensitivity and low cost, their high false positive rate can cause confusion in the patients and clinicians until a more specific test is done. OraQuick Advance Rapid HIV-1/2 Antibody Test (OraQuick) (OraSure Technologies, USA) is a rapid test that can detect HIV-1/2 antibodies in 20 minutes. It uses oral fluid, whole blood or serum sample. In this study, we evaluated the usefulness of the OraQuick as a screening and point-of-care test for HIV infection. Methods: From Jan 2007

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Three Adult Cases of Elizabethkingia meningoseptica Infection in a Korean Hospital

Case report PDF Jayoung Kim1, Yeon-Joon Park1, Soyoung Shin1, Yonggoo Kim1, Sun Hee Park2 Departments of 1Laboratory Medicine, 2Internal Medicine, College of Medicine, The Catholic University of Korea, Seoul, Korea Corresponding to Yeon-Joon Park, E-mail: yjpk@catholic.ac.kr Ann Clin Microbiol 2011;14(4):153-157. https://doi.org/10.5145/KJCM.2011.14.4.153Copyright © Korean Society of Clinical Microbiology. Abstract Elizabethkingia meningoseptica (Chryseobacterium meningoseptica) is a ubiquitous Gram-negative bacillus in the natural and hospital environments. This microorganism causes neonatal meningitis but rarely causes infections in adults, with most adult cases occurring in severely immunocompromised patients. Since E. meningoseptica is inherently resistant to the usual empiric therapy aimed at Gram-negative bacilli and MIC breakpoints for resistance and susceptibility of E. meningoseptica have not been established by the Clinical and Laboratory Standards Institute, it is very difficult to select effective antibiotics for the treatment of E. meningoseptica infection. We report here three cases of E. meningoseptica isolates (two from blood and one from CSF)

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Application of Matrix-Assisted Laser Desorption Ionization Time-of-Flight Mass Spectrometry to Screen the Extended-Spectrum β-Lactamase-Producing ST131 Escherichia coli Strains

Original article PDF Young Ah Kim1, Dongeun Yong2, Yong Ha In3, Hyung Soon Park3, Kyungwon Lee2 1Department of Laboratory Medicine, National Health Insurance Service Ilsan Hospital, Goyang, 2Department of Laboratory Medicine and Research Institute of Bacterial Resistance, Yonsei University College of Medicine, 3R&D Center, ASTA Inc, Suwon, Korea Corresponding to Dongeun Yong, E-mail: deyong@yuhs.ac Ann Clin Microbiol 2016;19(3):65-69. https://doi.org/10.5145/ACM.2016.19.3.65Copyright © Korean Society of Clinical Microbiology. Abstract Background: Sequence type 131 (ST131) O25b serogroup Escherichia coli, producing CTX-M type extended-spectrum β-lactamase (ESBL), is a major clone involved in worldwide pandemic spread in both community- and healthcare-associated infections. Recently, matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) has become a routine tool for the identification of bacteria in many laboratories. This study aimed to assess the performance of MALDI-TOF MS for the screening of ESBL-producing E. coli ST131 in a rapid, inexpensive, and simple way. Methods: A total 26 clinical E.

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Evaluation of Diagnostic Performance of Three Real-Time PCR Assays for the Detection of Mycobacteria Species

Original article Sang-wook Kim1*, Young-Hee Park2*, Young Jin Ko1,3, Yoon Ho Kim2, Chang Hyun Kim2, Chae Seung Lim1 Department of Laboratory Medicine, 1Korea University College of Medicine, Seoul, 2Korea University Medical Center Guro Hospital, Seoul, 3Department of Laboratory Medicine, College of Medicine, Chosun University, Gwangju, Korea *These authors contributed equally to this work. Corresponding to Young Jin Ko, E-mail: yjko@chosun.ac.kr Ann Clin Microbiol 2020;23(2):93-104. https://doi.org/10.5145/ACM.2020.23.2.6Received on 19 November 2019, Revised on 29 December 2019, Accepted on 29 December 2019, Published on 20 June 2020.Copyright © Korean Society of Clinical Microbiology. Abstract Background: The disease burden caused by Mycobacterium tuberculosis (MTB) complex continues to decrease in most countries. However, the diseases caused by the nontuberculous mycobacteria (NTM) become a public health problem. This study aimed to compare the diagnostic accuracy of three real-time PCR assays: AdvanSure TB/NTM real-time PCR kit (AdvanSure; LG Chem., Korea), Genedia MTB/NTM detection kit (Genedia; Green Cross

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Comparison of Three Methods with CHROMagar for Surveillance Culture of Carbapenem-Resistant Acinetobacter baumannii

Original article Kibum Jeon1, Seung Soon Lee2, Hyun Soo Kim1, Jae-Seok Kim1, Young Kyung Lee1, Wonkeun Song1, Han-Sung Kim1 1Department of Laboratory Medicine, 2Division of Infectious Diseases, Hallym University College of Medicine, Anyang, Korea Corresponding to Han-Sung Kim, E-mail: kimhs@hallym.or.kr Ann Clin Microbiol 2020;23(2):67-72. https://doi.org/10.5145/ACM.2020.23.2.3Received on 24 July 2019, Revised on 15 November 2019, Accepted on 15 November 2019, Published on 20 June 2020.Copyright © Korean Society of Clinical Microbiology. Abstract Background: Carbapenem-resistant Acinetobacter baumannii (CRAB) has emerged as an important nosocomial pathogen.The purpose of this study was to determine the effective methods for performing surveillance cultures of CRAB. Methods: Nasal and rectal swabs were obtained concurrently from hospitalized intensive care unit patients colonized with CRAB. All the samples were inoculated in CHROMagar Acinetobacter medium with CR102 (CHROMagar), MacConkey agar medium supplemented with 5 µg/mL imipenem (MCA-IPM), and triptic soy broth medium supplemented with 5 µg/ mL imipenem (TSB-IPM). CRAB

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Evaluation of Five Automated Urine Analyzers as Screening Instruments for Enhancing Diagnostic Efficiency in Urinary Tract Infection

Original article Jooyoung Cho1,2*, Jung-Hyun Byun1,3,4*, Sang-Guk Lee1, Kyeongjin Oh1, Beomchan Jeon1, Dongeun Yong1,3, Jeong-Ho Kim1,5 1Department of Laboratory Medicine, Severance Hospital, Yonsei University College of Medicine, Seoul, 2Department of Laboratory Medicine, Wonju Severance Christian Hospital, Yonsei University Wonju College of Medicine, Wonju, 3Research Institute of Bacterial Resistance, Yonsei University College of Medicine, Seoul, 4Department of Laboratory Medicine, Gyeongsang National University Hospital, Gyeongsang National University College of Medicine, Jinju, 5Department of Laboratory Medicine, Yongin Severance Hospital, Yonsei University College of Medicine, Yongin, Korea *These authors contributed equally to this work. Corresponding to Dongeun Yong, E-mail: deyong@yuhs.ac; Jeong-Ho Kim, E-mail: jeongho@yuhs.ac Ann Clin Microbiol 2021;24(3):83-96. https://doi.org/10.5145/ACM.2021.24.3.3Received on 1 April 2021, Revised on 15 July 2021, Accepted on 19 July 2021, Published on 20 September 2021.Copyright © Korean Society of Clinical Microbiology. Abstract Background: Although urine culture is considered a reference standard for the diagnosis of urinary tract infection (UTI), it is

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Four genotypes of carbapenem-resistant Acinetobacter baumannii strains lacking OXA-23 production in Korea

Brief communication Young Ah Kim1, Seok Hoon Jeong2,3, Jong Hee Shin4, Kyeong Seob Shin5, Jeong Hwan Shin6, Young Ree Kim7, Hyun Soo Kim8, Young Uh9, Nam Hee Ryoo10 1Department of Laboratory Medicine, National Health Insurance Service Ilsan Hospital, Goyang, 2Department of Laboratory Medicine, Yonsei University College of Medicine, Seoul, 3Research Institute of Bacterial Resistance, Yonsei University College of Medicine, Seoul, 4Department of Laboratory Medicine, Chonnam National University Medical School, Gwangju, 5Department of Laboratory Medicine, Chungbuk National University College of Medicine, Cheongju, 6Department of Laboratory Medicine and Paik Institute for Clinical Research, Inje University College of Medicine, Busan, 7Department of Laboratory Medicine, Jeju National University, College of Medicine, Jeju, 8Department of Laboratory Medicine, Hallym University Dongtan Sacred Heart Hospital, Hallym University College of Medicine, Hwaseong, 9Department of Laboratory Medicine, Yonsei University Wonju College of Medicine, Wonju, 10Department of Laboratory Medicine, Keimyung University School of Medicine, Daegu, Korea Corresponding to Young Ah

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Patterns of Antimicrobial Susceptibility of the Causative Bacteria of Urinary Tract Infections in Recent Years in an Island Region

Original article PDF Young Ree Kim1, Jung-Sik Huh2, Sung-Ha Kang1 Departments of 1Laboratory Medicine and 2Urology, Cheju National University College of Medicine, Jeju, Korea Corresponding to Young Ree Kim, E-mail: namu8790@empal.com Ann Clin Microbiol 2007;10(1):19-24.Copyright © Korean Society of Clinical Microbiology. Abstract Background: In order to provide a guideline for empirical treatment of urinary tract infections, we studied a change in causative organisms and antimicrobial susceptibility in our region of an island. Methods: We reviewed the results of antimicrobial susceptibility and the hospital charts of 3,064 patients with a significant bacteriuria (more than 105 colony forming unit/mL in urine cultures); the patients had been admitted to or seen at the out-patient clinic of Cheju University Hospital during the period from January 2002 to December 2005. Results: The most common pathogens were Escherichia coli (44.9%), Klebsiella spp. (8.1%), and Pseudomonas spp. (7.0%). In E. coli, the mean percent resistance to ampicillin, trimethoprim-sulfamethoxazole, and ciprofloxacin during the 4-year period was 69.0%, 32.5%, and

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