Table 4. Results of AdvanSure plus PCR, AdvanSure PCR assay and AFB staining according to the results of culture
Ann Clin Microbiol 2020;23:105-116. Evaluation of the AdvanSure TB/NTM Plus Real-Time PCR Assay for the Simultaneous Detection of Mycobacterium tuberculosis and Nontuberculous Mycobacteria from Clinical Specimens Download table Culture MTC (n=45) No. (%) Negative (n=129) No. (%) NTM (n=25) No. (%) AdvanSure PCR MTC 45 (100) 25 (19.4) 0 Negative 0 […]
Table 3. Results of AdvanSure plus PCR and AdvanSure PCR in 7 cases of MTC and NTM mix
Ann Clin Microbiol 2020;23:105-116. Evaluation of the AdvanSure TB/NTM Plus Real-Time PCR Assay for the Simultaneous Detection of Mycobacterium tuberculosis and Nontuberculous Mycobacteria from Clinical Specimens Download table Species AdvanSure Plus PCR AdvanSure PCR MTC Ct NTM Ct IC Ct Result MTC Ct NTM Ct IC Ct Result M. intracellulare 33.57 17.87 24.06 MTC and […]
Table 2. Performance of AdvanSure plus PCR in 200 mycobacterial strains.
Ann Clin Microbiol 2020;23:105-116. Evaluation of the AdvanSure TB/NTM Plus Real-Time PCR Assay for the Simultaneous Detection of Mycobacterium tuberculosis and Nontuberculous Mycobacteria from Clinical Specimens Download table Culture AdvanSure Plus PCR MTC (n=97) MTC and NTM (n=7) NTM(n=96) MTC 97 3 0 NTM 0 4 96 M. abscessus 0 0 14 […]
Table 1. Frequency of Mycobacterium species in this study
Ann Clin Microbiol 2020;23:105-116. Evaluation of the AdvanSure TB/NTM Plus Real-Time PCR Assay for the Simultaneous Detection of Mycobacterium tuberculosis and Nontuberculous Mycobacteria from Clinical Specimens Download table Species No. % MTC 100 50.0 NTM 100 50.0 M. abscessus 14 6.2 M. avium species 28 12.4 M. kansasii 4 […]
Table 6. Diagnostic accuracy of three real-time PCR assays for NTM detection stratified by AFB smear results (n=261)
Ann Clin Microbiol 2020;23:93-104. Evaluation of Diagnostic Performance of Three Real-Time PCR Assays for the Detection of Mycobacteria Species Download table Parameter AFB smear AdvanSure Genedia PowerChek Result 95% CI Result 95% CI Result 95% CI Sensitivity (%) Positive 90.9 70.8-98.9 54.6 32.2-75.6 95.5 77.2-99.9 Negative 40.0 5.3-85.3 0.0 0.0-52.2 60.0 14.7-94.7 Total 81.5 61.9-93.7 […]
Table 5. Diagnostic accuracy of three real-time PCR assays for the MTB detection stratified by AFB smear results (n=261)
Ann Clin Microbiol 2020;23:93-104. Evaluation of Diagnostic Performance of Three Real-Time PCR Assays for the Detection of Mycobacteria Species Download table Parameter AFB smear AdvanSure Genedia PowerChek Result 95% CI Result 95% CI Result 95% CI Sensitivity (%) Positive 98.4 91.5-100.0 96.8 89.0-99.6 98.4 91.5-100.0 Negative 0.0 0.0-79.3 0.0 0.0-79.3 0.0 0.0-79.3 Total 96.9 89.2-99.6 […]
Table 4. Agreement between AFB culture tests and three real-time PCR assays for MTB and NTM detection (n=261)
Ann Clin Microbiol 2020;23:93-104. Evaluation of Diagnostic Performance of Three Real-Time PCR Assays for the Detection of Mycobacteria Species Download table Parameter Target organism AdvanSure Genedia PowerChek Result 95% CI Result 95% CI Result 95% CI PPA (%) MTB 96.9 89.3-99.1 95.3 87.1-98.4 96.9 89.3-99.1 NTM 81.8 65.6-91.4 44.4 27.6-62.7 88.9 71.9-96.1 PNA (%) MTB […]
Table 3. Discrepant results between three real-time PCR assays and AFB culture in AFB smear negative specimens
Ann Clin Microbiol 2020;23:93-104. Evaluation of Diagnostic Performance of Three Real-Time PCR Assays for the Detection of Mycobacteria Species Download table Specimen type AdvanSure Genedia PowerChek AFB culture Sputum NTM Negative NTM No growth Bronchial aspirate NTM Negative NTM NTM Sputum NTM Negative NTM NTM Pleural fluid MTB Negative MTB NTM Sputum Negative Negative NTM […]
Table 2. Discrepant results among three real-time PCR assays and AFB culture in AFB smear positive specimens
Ann Clin Microbiol 2020;23:93-104. Evaluation of Diagnostic Performance of Three Real-Time PCR Assays for the Detection of Mycobacteria Species Download table Specimen type (No.) AdvanSure Genedia PowerChek No. of specimens AFB culture results Sputum (4), Bronchial aspirate (9) NTM Negative NTM 13 NTM Sputum (6), Bronchial aspirate (1) MTB MTB MTB 7 No growth Sputum […]
Table 1. The AFB culture results for the specimens of AFB smear negative and MTB/NTM real-time PCR negative
Ann Clin Microbiol 2020;23:93-104. Evaluation of Diagnostic Performance of Three Real-Time PCR Assays for the Detection of Mycobacteria Species Download table Specimen Results MTB NTM Negative Subtotal (%) Sputum 0 3 68 71 (40.1) Bronchial aspirate 0 2 46 48 (27.1) Pleural fluid 1 1 25 27 (15.3) Cerebrospinal fluid (CSF) 0 0 16 16 […]