Original article PDF Soon Deok Park1, Young Uh1, In Ho Jang1, Ohgun Kwon1, Kap Jun Yoon1, Hyo Youl Kim2 Departments of 1Laboratory Medicine and 2Infectious Diseases, Yonsei University Wonju College of Medicine, Wonju, Korea Corresponding to Soon Deok Park, E-mail: mizpark66@empal.com Ann Clin Microbiol 2009;12(1):24-29.Copyright © Korean Society of Clinical Microbiology. Abstract Background: Accurate detection of organisms producing extended-spectrum β-lactamase (ESBL) and AmpC β-lactamase is very important for treatment of patients. However, unlike the ESBL confirmatory test, there are no guidelines for detection of organisms producing AmpC β-lactamase. We evaluated a detection method using boronic acid (BA) for ESBL and AmpC β-lactamase.  Methods: Clinical isolates of Escherichia coli, Klebsiella pneumoniae, Klebsiella oxytoca, and Proteus mirabilis showing intermediate resistance or resistance to cefoxitin (FOX) or positive for ESBL were tested. A ≥5 mm increase in zone diameter of ceftazidime/clavulanic acid/BA (CAZ/CA/BA) and/or cefotaxime/clavulanic acid/BA (CTX/CA/BA) versus CAZ/BA and/or CTX /BA was considered positive for ESBL. Likewise, a

Original article PDF Soon Deok Park1, Young Uh1, In Ho Jang1, Kap Jun Yoon1, Jong Hee Shin2 Department of Laboratory Medicine, 1Yonsei University Wonju College of Medicine, Wonju, 2Chonnam National University Medical School, Gwangju, Korea Corresponding to Young Uh, E-mail: u931018@yonsei.ac.kr Ann Clin Microbiol 2010;13(3):114-120. https://doi.org/10.5145/KJCM.2010.13.3.114Copyright © Korean Society of Clinical Microbiology. Abstract Background: The VITEK-2 yeast susceptibility test (AST-YS01; bioMerieux, Hazelwood, MO, USA) has recently been introduced as a fully automated, commercial antifungal susceptibility test system that determines MIC endpoints spectrophotometrically, thereby eliminating subjective errors. We compared the ATB FUNGUS 2 (bioMerieux) and VITEK-2 (AST- YS01) systems to the CLSI M27 method for susceptibility testing of Candida isolates.  Methods: We tested 59 Candida species that were isolated from blood cultures at Wonju Christian Hospital between September 2008 and August 2009. We compared MIC results for amphotericin B, flucytosine, fluconazole and voriconazole using the ATB FUNGUS 2 and VITEK-2 (AST-YS01)

Case report PDF Min Jin Kim1, So Young Kim1, Yong Ho Park1, Hoi Soo Yoon2, Jin-Tae Suh1, Hee Joo Lee1 Departments of 1Laboratory Medicine, 2Pediatrics, Kyung Hee University School of Medicine, Seoul, Korea Corresponding to Hee Joo Lee, E-mail: leehejo@khmc.or.kr Ann Clin Microbiol 2011;14(3):110-114. https://doi.org/10.5145/KJCM.2011.14.3.110Copyright © Korean Society of Clinical Microbiology. Abstract Campylobacter jejuni is one of the important bacterial pathogens causing entero-invasive diarrhea; however, C. jejuni infection is rarely complicated by bacteremia or extra-intestinal localization. In the domestic literature, the majority of the relevant reports have focused on Campylobacter fetus, which causes bacteremia more frequently than enteritis, but there are no reports of C. jejuni bacteremia in Korea. We present the case of a 13-year-old girl who presented with abdominal pain. Blood cultures revealed curved Gram-negative bacilli and small, mucoid, gray colonies on blood agar plates at 37oC. Biochemical tests showed oxidase-positive colonies. To confirm the species, 16S rRNA

Original article PDF Young Uh1,2, In Ho Jang1, Soon Deok Park1, Kab Seung Kim1, Dong Min Seo2, Kap Jun Yoon1, Hee Kyoung Choi3, Young Keun Kim3, Hyo Youl Kim3 Departments of 1Laboratory Medicine, 2Medical Information Development and 3Internal Medicine, Yonsei University Wonju Severance Christian Hospital, Wonju, Korea Corresponding to Young Uh, E-mail: u931018@yonsei.ac.kr Ann Clin Microbiol 2014;17(2):58-64. https://doi.org/10.5145/ACM.2014.17.2.58Copyright © Korean Society of Clinical Microbiology. Abstract Background: The false positive signals of a continuous monitoring blood culture system (CMBCS) increase the reporting time and laboratory cost. This study aimed to determine the highly relevant variables that discriminate false positive signals from true positive signals in a CMBCS. Methods: Among 184,363 blood culture sets (aerobic and anaerobic), the signal-positive samples according to a BACTEC FX system (Plus Aerobic/F, BDA; Plus Anaerobic/F, BDN) and BacT/Alert 3D system (Standard Aerobic, BSA; Standard Anaerobic, BSN) between April 2010 and November 2013 were classified into two

Research note PDF Hyeun Gyeo Lee1, Gyu Yel Hwang1, Soon Deok Park1, Young Uh1, Juwon Kim1, Kap Jun Yoon1, Won-Yeon Lee2 Departments of 1Laboratory Medicine and 2Internal Medicine, Yonsei University Wonju College of Medicine, Wonju, Korea Corresponding to Young Uh, E-mail: u931018@yonsei.ac.kr Ann Clin Microbiol 2015;18(2):52-55. https://doi.org/10.5145/ACM.2015.18.2.52Copyright © Korean Society of Clinical Microbiology. Abstract A total of 1,132 pleural fluid culture results obtained from October 2012 to July 2014 were analyzed to elucidate the microbiological characteristics according to transudative and exudative pleural fluid. The pleural fluid cultures were performed using aerobic and anaerobic blood culture bottles. The blood and pleural fluid for total protein, lactate dehydrogenase, and glucose measurement were submitted to laboratory at the same time with pleural fluid cultures. The rates for culture positivity, anaerobes isolation, and polymicrobials between transudative and exudative pleural fluid were 5.2% vs. 10.4%, 14.8% vs. 7.8%, and 14.8% vs. 10.9%. (Ann Clin Microbiol