Original article PDF Young Uh*, Soon Deok Park, Jeong Seog Son*, Hyeun Gyeo Lee*, An Suk Jeoung*, Hyun Mi Cho*, and Kap Jun Yoon*, and Hyo Youl Kim Departments of Clinical Pathology* and Internal Medicine**, Yonsei University Wonju College of Medicine, Wonju, and Research Institute of Bacterial Resistance***, Yonsei University College of Medicine, Seoul, Korea Corresponding to Young Uh Ann Clin Microbiol 2000;3(2):99-110.Copyright © Korean Society of Clinical Microbiology. Abstract Background: In recent years, the incidence of extended-spectrum β-lactamase (ESBL) producing Klebsiella has been steadily increased, and the newer species K. planticola and K. terrigena, formerly regarded as nonpathogen, have been reported with astonishing frequency from human infectious processes by some investigators. The aim of this study is to elucidate the isolation rate and antimicrobial susceptibility of recent clinical Klebsiella isolates. Method: For the clinical Klebsiella isolates during the period of June 1999 to May 2000, isolation frequency of Klebsiella species by specimen, departments, age, and sex were analyzed. And antimicrobial susceptibilities

Original article PDF Young Uh, Gyu Yel Hwang, In Ho Jang, Hyeun Gyeo Lee, An Suk Jeoung, Soon Deok Park, Jeong Seog Son, and Kap Jun Yoon Department of Clinical Pathology, Yonsei University Wonju College of Medicine, Wonju, Korea Corresponding to Young Uh, E-mail: u931018@wonju.yonsei.ac.kr Ann Clin Microbiol 2001;4(1):22-27.Copyright © Korean Society of Clinical Microbiology. Abstract Background: Although most of aerobic gram-positive bacilli have been considered to be contaminants, gram-positive bacilli should be identified to the species level if they are isolated from sterile body sites such as blood, and from adequately collected clinical specimens if they are the predominant organisms. However, identification of gram-positive bacilli are difficult due to the enormous diversity of these organisms and the small number of readily available commercial identification systems in clinical laboratories. Gram-positive bacilli and coccorods isolated from blood cultures were tested with BBL Crystal Gram-Positive (GP) Identification (ID) system in order to

Original article PDF Young Uh, Soon Duk Park, Hyun Mi Cho, Hyeun Gyeo Lee, An Suk Jeoung, Mi Kyung Lee, and Kap Jun Yoon Department of Clinical Pathology, Yonsei University Wonju College of Medicine, Wonju, Korea Corresponding to Young Uh, E-mail: u931018@wonju.yonsei.ac.kr Ann Clin Microbiol 2002;5(1):42-46.Copyright © Korean Society of Clinical Microbiology. Abstract Background: The test for hippurate hydrolysis is critical for differentiation of C. jejuni and other thermophilic Campylobacter species. So, we evaluated the disk method for detection of hippurate hydrolysis by C. jejuni. Methods: Twenty-eight Campylobacter species isolated from stool culture were simultaneously tested with disk method for detection of hippurate hydrolysis and polymerase chain reaction (PCR) for hippuricase specific gene. Disk method was tested with difference in incubation time (2 hours vs. 4 hours), hippurate concentration (1%, 2%, and 4%), amount of ninhydrin (50 μL vs. 100 μL), and inoculation method (colony vs. suspension of organism adjusted by turbidity), finally, 24 types of disk

Case report PDF Young Uh1 , Soon Deok Park1, Gyu Yul Hwang1, Kap Jun Yoon1, Hwang Min Kim2, and Hyo Youl Kim3 Departments of Laboratory Medicine1, Pediatrics2 and Infectious Disease3, Yonsei University Wonju College of Medicine, Wonju, Korea Corresponding to Young Uh, E-mail: u931018@wonju.yonsei.ac.kr Ann Clin Microbiol 2004;7(2):186-189.Copyright © Korean Society of Clinical Microbiology. Abstract Verotoxin-producing Escherichia coli O157 is a primary cause of severe and bloody diarrhea. Campylobacter spp. are one of the commonly reported bacterial cause of gastrointestinal infections throughout the world. Only a few cases involving both E. coli O157 and Campylobacter species have been reported. The authors simultaneously isolated verotoxin-producing E. coli O157 and Campylobacter species from the stool of a 3 year-old male with bloody diarrhea, fever and abdominal pain. (Korean J Clin Microbiol 2004;7(2):186-189) Keywords Escherichia coli O157, Campylobacter, Gastroenteritis, Verotoxin

Case report PDF Sang Sun Hwang1, Soon Deok Park1, Ohgun Kwon1, Young Uh1, Kap Jun Yoon1, Sug-Won Kim2 Departments of 1Laboratory Medicine and 2Plastic Surgery, Yonsei University Wonju College of Medicine, Wonju, Korea Corresponding to Sang Sun Hwang, E-mail: 41269@hanmail.net Ann Clin Microbiol 2007;10(2):168-170.Copyright © Korean Society of Clinical Microbiology. Abstract Pasteurella dagmatis is an oxidase and catalase positive, facultative anaerobic, gram-negative coccobacillus classified as a member of the family Pasteurellaceae. Pasteurella species are commonly colonizing the oropharynx of healthy domestic and wild animals including cats and dogs. These are usually pathogenic to domestic animals, but rarely to human beings. Pasteurella infection of human causes pneumonia, empyema, meningitis, peritonitis, bone and joint infection and septicemia. Recently, we experienced a case of dog-bite wounds from which Pasteurella dagmatis was isolated in a 39-year-old woman. To our knowledge, this is the first report of Pasteurella dagmatis isolated from dog-bite wounds in Korea. (Korean J Clin Microbiol 2007;10:168-170) Keywords Pasteurella dagmatis, Dog-bite, Wound

Case report PDF Kyung Ran Jun1, Mi-Sook Jang1, Sook Ja Park1, Mi-Na Kim1, Dong Yoon Kim2, Hungwon Tchah2, Myoung Joon Kim2 Departments of 1Laboratory Medicine and 2Ophthalmology, University of Ulsan College of Medicine and Asan Medical Center, Seoul, Korea Corresponding to Mi-Na Kim, E-mail: mnkim@amc.seoul.kr Ann Clin Microbiol 2007;10(1):73-76.Copyright © Korean Society of Clinical Microbiology. Abstract Beauveria bassiana is a hyaline Hypomycetes, which is known as an insect pathogen causing infections in silkworm. It is a rare opportunistic pathogen of human accounted for pulmonary infection, keratitis, and deep tissue infection. We report the first case of B. bassiana keratitis in Korea. A 64-year-old man with a 10-year history of herpetic keratitis was referred for the treatment of infectious keratitis in the right eye. Corneal scrapings showed septate hyaline hyphae on calcoflour white-KOH preparation and their cultures grew B. bassiana. At the beginning, the patient was treated empirically with an antiviral and antibiotics, and then the treatment was changed with

Case report PDF Soon Deok Park1, Young Uh1, Hyeun Gyeo Lee1, Ih Ho Jang1, Kap Jun Yoon1, Mee Kyung Namgoong2 Departments of 1Laboratory Medicine and 2Pediatrics, Yonsei University Wonju College of Medicine, Wonju, Korea Corresponding to Young Uh, E-mail: u931018@yonsei.ac.kr Ann Clin Microbiol 2009;12(3):133-137.Copyright © Korean Society of Clinical Microbiology. Abstract Rothia dentocariosa, a pleomorphic gram-positive branching bacillus, is a common inhabitant of the nose and throat. It is a well-known causative agent of dental plaques and periodontal diseases. Although generally regarded as having a low virulence to humans, R. dentocariosa has been recognized as causative agents of infective endocarditis and bacteremia with increasing frequency. Consequently, it can be a very serious pathogen when isolated from usually sterile sites such as blood or cerebrospinal fluid. We report a case of Rothia dentocariosa bacteremia without endocarditis in a 17-month-old male patient with fever, vomiting and diarrhea. (Korean J Clin Microbiol 2009;12:133-137) Keywords Bacteremia, Rothia dentocariosa, Blood culture