Annals of Clinical Microbiology, The official Journal of the Korean Society of Clinical Microbiology

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Indexed in KCI, KoreaMed, Synapse, DOAJ
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pISSN 2288-0585 eISSN 2288-6850

Search Results for: Dokyun Kim

Evaluation of the Performance of ASTA MicroIDSys, a Novel Matrix-Assisted Laser Desorption/Ionization-Time of Flight Mass Spectrometry System, in Identification of Bacterial Clinical Isolates

Original article Changseung Liu1*, Eunjung Lee2*, Dokyun Kim1, Seok Hoon Jeong1 1Department of Laboratory Medicine and Research Institute of Bacterial Resistance, Yonsei University College of Medicine, Seoul, 2Department of Clinical Pathology, Sangji University College of Science, Wonju, Korea *These authors contributed equally to this work. Corresponding to Dokyun Kim, E-mail: kyunsky@yuhs.ac Ann Clin Microbiol 2020;23(3):135-148. https://doi.org/10.5145/ACM.2020.23.3.3Received on 31 January 2020, Revised on 10 April 2020, Accepted on 10 April 2020, Published on 20 September 2020.Copyright © Korean Society of Clinical Microbiology. Abstract Background: We evaluated the performance of ASTA MicroIDSys (ASTA, Korea) and Bruker Biotyper (Bruker Daltonics, Germany) systems in the identification of bacterial isolates from clinical microbiology laboratory specimens during the study period. In addition, species for which the identification accuracy using MALDI-TOF MS systems was previously reported to be poor were also identified by comparing the MS results with those obtained using molecular identification. Methods: A total of

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Clinical and microbiological risk factors for severe Clostridioides difficile infections

Original article Young Ah Kim1, Heejung Kim2,3, Dokyun Kim3, Changseung Liu3, Seok Hoon Jeong3 1Department of Laboratory Medicine, National Health Insurance Service Ilsan Hospital, Goyang, 2Department of Laboratory Medicine, Yongin Severance Hospital, YongIn, 3Department of Laboratory Medicine and Research Institute of Bacterial Resistance, Yonsei University College of Medicine, Seoul, Korea Corresponding to Heejung Kim, E-mail: hjkim12@yuhs.ac Ann Clin Microbiol 2022;25(1):17-23. https://doi.org/10.5145/ACM.2022.25.1.3Received on 16 February 2022, Revised on 8 March 2022, Accepted on 14 March 2022, Published on 20 March 2022.Copyright © Korean Society of Clinical Microbiology.This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (https://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. Abstract Background: There has been a marked increase in the mortality rate associated with Clostridioides difficile infection (CDI) globally since 2003, with the emergence of binary toxinproducing ribotype 027

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Clinical and microbiological risk factors for community-associated Clostridioides difficile infections

Original article Young Ah Kim1, Heejung Kim2, Dokyun Kim2, Changseung Liu3, Seok Hoon Jeong2 1Department of Laboratory Medicine, National Health Insurance Service, Ilsan Hospital, Goyang, 2Department of Laboratory Medicine and Research Institute of Bacterial Resistance, Yonsei University College of Medicine, Seoul, 3Department of Laboratory Medicine, Gangneung Asan Hospital, University of Ulsan College of Medicine, Gangneung, Korea Corresponding to Heejung Kim, E-mail: hjkim12@yuhs.ac Ann Clin Microbiol 2022;25(2):47-52. https://doi.org/10.5145/ACM.2022.25.2.3Received on 15 March 2022, Revised on 27 April 2022, Accepted on 27 April 2022, Published on 20 June 2022.Copyright © Korean Society of Clinical Microbiology.This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (https://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. Abstract Background: The incidence of community-associated (CA) Clostridioides difficile infection (CDI) has increased in Korea. In this study, we evaluated CA-CDI risk factors

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Nationwide Survey of Blood Culture Protocol in Clinical Microbiology Laboratories in Korea

Original article PDF Young Ah Kim1, Dokyun Kim2, Dongeun Yong2, Kyungwon Lee2 1Department of Laboratory Medicine, National Health Insurance Service Ilsan Hospital, Goyang, 2Department of Laboratory Medicine and Research Institute of Bacterial Resistance, Yonsei University College of Medicine, Seoul, Korea Corresponding to Dongeun Yong, E-mail: deyong@yuhs.ac Ann Clin Microbiol 2016;19(4):97-104. https://doi.org/10.5145/ACM.2016.19.4.97Copyright © Korean Society of Clinical Microbiology. Abstract Background: The recovery of bacteria from blood can be affected by many factors. Standardization of blood culture methods is important for reliability. Herein, we aimed to investigate blood culture protocols in Korea. Methods: We performed a multicenter survey with a questionnaire about blood culture practices, which was sent by email to directors and clinical physicians in charge of clinical microbiology laboratories in May 2014. Total data from 18 participating hospitals were analyzed to be used as current baseline data, which is necessary to optimize blood culture protocols. Results: Many laboratories included recommended

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Performance Evaluation of Newly Developed Korean Antimicrobial Susceptibility Testing Panels for MicroScan System Using Clinical Isolates from Teaching Hospitals in Korea

Original article PDF Da Young Kang1,2, Jun Sung Hong1, Byeol Yi Park1,2, In-Ho Jang2, Dokyun Kim1, Hyukmin Lee1, Seok Hoon Jeong1 1Department of Laboratory Medicine and Research Institute of Bacterial Resistance, Yonsei University College of Medicine, Seoul, 2Department of Clinical Pathology, Sangji University College of Health Science, Wonju, Korea Corresponding to Dokyun Kim, E-mail: kyun-sky@yuhs.ac Ann Clin Microbiol 2019;22(3):61-70. https://doi.org/10.5145/ACM.2019.22.3.61Copyright © Korean Society of Clinical Microbiology. Abstract Background: Antimicrobial resistant continues to pose a threat to public health. Therefore, rapid and accurate antimicrobial susceptibility testing is very important. The objectives of this study were to evaluate the performance of the MicroScan system (Beckman Coulter, USA) with newly developed Korean Antimicrobial Susceptibility Testing Panels (KSCM panels) for antimicrobial susceptibility testing (AST) against clinical isolates in South Korea. Methods: Three KSCM panels were designed in this study. For the performance evaluation, a total of 1,325 clinical isolates including 1,027 of Gram-negative bacilli

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Performance Evaluation of SENTiFIT 270 and FOB Gold Reagent for Detecting Fecal Occult Blood

Original article PDF Da Young Kang1,2, Dokyun Kim1, Keonhan Kim1, In-Ho Jang2, Seok Hoon Jeong1 1Department of Laboratory Medicine and Research Institute of Bacterial Resistance, Yonsei University College of Medicine, Seoul, 2Department of Clinical Pathology, Sangji University College of Health Science, Wonju, Korea Corresponding to Dokyun Kim, E-mail: kyunsky@yuhs.ac Ann Clin Microbiol 2019;22(2):29-34. https://doi.org/10.5145/ACM.2019.22.2.29Copyright © Korean Society of Clinical Microbiology. Abstract Background: Fecal occult blood tests have been widely used to screen for colorectal cancer. SENTiFIT 270 (Sentinel diagnostics, Italy) is a fecal occult blood test with an immunochemical method that utilizes FOB Gold reagents. We evaluated the performance of SENTiFIT 270 using the FOB Gold reagent. In addition, FOB Gold was evaluated with the HITACHI 7180 (Hitachi Ltd., Japan). Methods: The precision and linearity of the SENTiFIT 270 was evaluated in accordance with applicable Clinical and Laboratory Standard Institute guidelines. The comparison study between SENTiFIT 270-FOB Gold and the OC-Sensor

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Differences in Antimicrobial Resistance Phenotypes by the Group of CTX-M Extended-Spectrum β-Lactamase

Original article PDF Bareum Gwon1,2, Eun-Jeong Yoon2, Dokyun Kim2, Hyukmin Lee2, Jong Hee Shin3, Jeong Hwan Shin4, Kyeong Seob Shin5, Young Ah Kim6, Young Uh7, Hyun Soo Kim8, Young Ree Kim9, Seok Hoon Jeong2 1Department of Clinical Pathology, Sangji University College of Science, Wonju, 2Department of Laboratory Medicine and Research Institute of Bacterial Resistance, Yonsei University College of Medicine, Seoul, 3Department of Laboratory Medicine, Chonnam National University School of Medicine, Gwangju, 4Department of Laboratory Medicine, Inje University Busan Paik Hospital, Busan, 5Department of Laboratory Medicine, College of Medicine, Chungbuk National University, Cheongju, 6Department of Laboratory Medicine, National Health Insurance Service Ilsan Hospital, Goyang, 7Department of Laboratory Medicine, Wonju Severance Christian Hospital, Yonsei University Wonju College of Medicine, Wonju, 8Department of Laboratory Medicine, Hallym University College of Medicine, Hwaseong, 9Department of Laboratory Medicine, School of Medicine, Jeju National University, Jeju, Korea Corresponding to Eun-Jeong Yoon, E-mail: ejyoon@yuhs.ac Ann Clin Microbiol 2019;22(1):1-8. https://doi.org/10.5145/ACM.2019.22.1.1Copyright © Korean Society of Clinical Microbiology. Abstract

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Evaluation of the Performance of ASTA MicroIDSys, a Novel Matrix-Assisted Laser Desorption/Ionization-Time of Flight Mass Spectrometry System, in Identification of Bacterial Clinical Isolates

PDF Original article Annals of Clinical Microbiology (Ann Clin Microbiol) 2020 September, Volume 23, Issue 3, pages 135-148. https://doi.org/10.5145/ACM.2020.23.3.3 Changseung Liu1*, Eunjung Lee2*, Dokyun Kim1 , Seok Hoon Jeong11Department of Laboratory Medicine and Research Institute of Bacterial Resistance, Yonsei University College of Medicine, Seoul, 2Department of Clinical Pathology, Sangji University College of Science, Wonju, Korea Abstract Background: We evaluated the performance of ASTA MicroIDSys (ASTA, Korea) and Bruker Biotyper (Bruker Daltonics, Germany) systems in the identification of bacterial isolates from clinical microbiology laboratory specimens during the study period. In addition, species for which the identification accuracy using MALDI-TOF MS systems was previously reported to be poor were also identified by comparing the MS results with those obtained using molecular identification. Methods: A total of 889 non-duplicated clinical isolates were included in this study. The results of ASTA MicroIDSys were compared with those of Bruker Biotyper; 16S rRNA sequencing was performed

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Epidemiological Study of an Outbreak of KPC-2-producing Klebsiella pneumoniae in a Tertiary Hospital in Korea

Original article Jun Sung Hong1*, Byeol Yi Park1*, Dokyun Kim1, Kunhan Kim1, Kyoung Hwa Lee2, Nan Hyoung Cho3, Seok Hoon Jeong1 1Department of Laboratory Medicine and Research Institute of Bacterial Resistance, Yonsei University College of Medicine, Seoul, 2Division of Infectious Disease, Department of Internal Medicine, Yonsei University College of Medicine, Seoul, 3Department of Infection Control, Gangnam Severance Hospital, Seoul, Korea. * These authors contributed equally to this work. Corresponding to Dokyun Kim, E-mail: kyunsky@yuhs.ac Ann Clin Microbiol 2020;23(2):81-92. https://doi.org/10.5145/ACM.2020.23.2.5Received on 23 October 2019, Revised on 6 January 2020, Accepted on 6 January 2020, Published on 20 June 2020.Copyright © Korean Society of Clinical Microbiology. Abstract Background: The prevalence of carbapenemase-producing Enterobacteriaceae (CPE), especially the KPC-2-producing Klebisella pneumoniae, is rapidly increasing and becoming a menace to global public health. This study aims to present the molecular epidemiology of the KPC-2-producing K. pneumoniae isolates emerged in a tertiary hospital in South Korea

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