Fig. 1. Functionality of Trichomonas vaginalis actin LAMP assays. (A) LAMP on 10-fold serial dilutions of T. vaginalis genomic DNA (10 ng to 1 pg per reaction) monitored by measuring absorbance. Distilled water was used as a negative control. (B) LAMP products were visualized by gel electrophoresis. Lane 1, 1 ng; Lane 2, 100 pg; Lane 3, 10 pg; Lane 4, 1 pg of T. vaginalis genomic DNA; Lane 5, LAMP product after HindIII digestion; Lane 6, distilled water; andLane M, 100-bp DNA marker. (C) LAMP products were visualized under UV light using Loopamp fluorescent detection reagent. Lane 1, 1 ng; Lane 2, 100 pg; Lane 3, 10 pg; Lane 4, 1 pg; Lane 5, 100 fg; Lane 6, 10 fg of T. vaginalis genomic DNA; and Lane 7, distilled water. (D and E) T. vaginalis at a density of 1×102 parasites/μL was serially diluted and tested using LAMP assay (D) and PCR (E) with F3 and B3 primers (Table 1). Lane M, 100-bp DNA marker; Lane 1, 100; Lane 2, 10; Lane 3, 1; Lane 4, 0.1; Lane 5, 0.01 parasite (s) per reaction; Lane 6, positive control, 100 pg of plasmid DNA containing LAMP targeting regions of actin gene; and Lane 7, distilled water. (F) Specificity of LAMP primers for detection of T. vaginalis assessed using template DNA from other microbial species. Lane 1, T. vaginalis; Lane 2, Candida albicans; Lane 3, Chlamydia trachomatis; Lane 4, Neisseria gonorrhoeae; Lane 5, Cryptosporidium parvum; Lane 6, Entamoeba histolytica; Lane 7, Giardia lamblia; Lane 8, Escherichia coli; and Lane 9, human genomic DNA. LAMP products were visualized via color change that was also observable by the naked eye under normal visible light reproduced under the CC-BY-NC license from the original article [19].
Ann Clin Microbiol 2024;27:69-79. Molecular diagnosis of parasitic diseases in Korea Download image
Fig. 5. The nematode eggs (Group IV). A. Ascaris lumbricoides (F); B. A. lumbricoides (F) in CTS specimen; C. A. lumbricoides (U); D. A. lumbricoides (U) in CTS specimen; E. Trichuris trichiura; F. T. trichiura in CTS specimen; G. Enterobius vermicularis in CTS specimen; H. E. vermicularis in a specimen of cellotape anal swab; I. Hookworm; J. Hookworm (morular stage) in CTS specimen; K. Air-bubble like hookworm egg of which a mature larva already escaped; L. Trichostorongylus orientalis. Scale bar = 25 ㎛. CTS, cellophane thick smear.
Ann Clin Microbiol 2024;27:49-67. Practical guide for the diagnosis of helminth ova in stools Download image
Fig. 4. The embryonated (cestode) eggs without an operculum (Group III). A. Taenia sp. in CTS specimen; B. Hymenolepis nana; C. H. nana in CTS specimen; D. Hymenolepis diminuta. Scale bar = 15 ㎛. CTS, cellophane thick smear.
Ann Clin Microbiol 2024;27:49-67. Practical guide for the diagnosis of helminth ova in stools Download image
Fig. 3. The unembryonated large-sized eggs with an operculum (Group II). A. Echinostomatid egg in CTS specimen; B. Isthmiophora hortensis; C. Echinostoma cinetorchis; D. Echinochasmus japonicus; E. Paragonimus westermani; F. Neodiplostomum seoulense; G. Fasciola hepatica; H. Dibothriocephalus nihonkaiensis. Scale bar = 50 ㎛. CTS, cellophane thick smear.
Ann Clin Microbiol 2024;27:49-67. Practical guide for the diagnosis of helminth ova in stools Download image
Fig. 2. The embryonated small-sized eggs with an operculum (Group I). A. Clonorchis sinensis; B. SEM-view of C. sinensis egg; C. Metagonimus yokogawai; D. Heterophyes nocens; E. Pygidiopsis summa; F. Stictodora fuscata; G. Plagiorchis muris; H. Eurytrema pancreaticum; I. Gymnophalloides seoi. Scale bar = 10 ㎛.
Ann Clin Microbiol 2024;27:49-67. Practical guide for the diagnosis of helminth ova in stools Download image
Fig. 1. Materials and procedures for the Kato-katz egg counting technique. (This figure was re-arranged based on Figs 1-8 [28])
Ann Clin Microbiol 2024;27:49-67. Practical guide for the diagnosis of helminth ova in stools Download image
Fig. 1. The experience with donor screening of Microbiotix Inc., a fecal microbiota bank in Korea (August 2017-June 2023). MDRO, multidrug-resistant organism.
Ann Clin Microbiol 2024;27:11-17. Screening for potential infections in fecal microbiota transplantation guidelines and an experience of microbiota bank operation in Korea: a narrative review Download image
Fig. 2. Antimicrobial resistance of group A streptococci against erythromycin (ERY), clindamycin (CLI), tetracycline (TET), and ofloxacin (OFX) isolated from Gyeongnam Province between 1995-2017.
Ann Clin Microbiol 2023;26:89-97. Clinical manifestations, diagnosis, and antimicrobial resistance of group A streptococci infections Download image
Fig. 1. Serotyping of group A streptococci by (a) T typing and (b) M typing.
Ann Clin Microbiol 2023;26:89-97. Clinical manifestations, diagnosis, and antimicrobial resistance of group A streptococci infections Download image
Fig. 2. The receiver operating characteristic (ROC) curves for the biomarkers evaluated in the present study. (a) ROC for predicting Gram negative bacteremia. (b) ROC for predicting Gram positive bacteremia. AUC, area under the curve; hsCRP, high sensitivity C-reactive protein; BUN, blood urea nitrogen
Ann Clin Microbiol 2023;26:103-115. Clinical usefulness of procalcitonin/albumin and blood urea nitrogen/albumin ratios for the early detection of bacteremia Download image