Pulmonary infection caused by Segniliparus rugosus in a patient without systemic immunodeficiency but with bronchiectasis: first identification by matrix-assisted laser desorption ionization time-of-flight mass spectrometry with whole-genome confirmation
Letter to the editor Jaesoon Kim1,2*, Nakwon Kwak3,4*, Jee-Soo Lee1,2, Taek Soo Kim1,2, Moon-Woo Seong1,2, Jae Hyeon Park1 1Department of Laboratory Medicine, Seoul National University Hospital, Seoul, Korea2Department of Laboratory Medicine, Seoul National University College of Medicine, Seoul, Korea3Division of Pulmonary and Critical Care Medicine, Department of Internal Medicine, Seoul National University Hospital, Seoul, Korea4Department […]
Table 3. PBP3 mutation profiles of β-lactamase-non-producing β-lactam-resistant Haemophilus influenzae
Ann Clin Microbiol 2025;28(4):23. Prevalence and molecular characteristics of β-lactam resistance in non-typeable Haemophilus influenzae isolates in Korea Download table Resistance profile Isolates (n = 16) TEM ftsI allele Amino acid substitutions D350 S357 M377 S385 L389 I449 A502 R517 N526 AM 2 – 40 N N I T F K AM, […]
Fig. 4. Distribution of negative samples by age group.
Ann Clin Microbiol 2025;28(4):25. Clinical evaluation of GenBody hMPV Ag Rapid Test: a diagnostic accuracy study Download image
Fig. 3. Distribution of positive samples by age group.
Ann Clin Microbiol 2025;28(4):25. Clinical evaluation of GenBody hMPV Ag Rapid Test: a diagnostic accuracy study Download image
Fig. 2. Distribution of sample count based on Ct value. Ct, cycle threshold.
Ann Clin Microbiol 2025;28(4):25. Clinical evaluation of GenBody hMPV Ag Rapid Test: a diagnostic accuracy study Download image
Fig. 1. Outline of the testing process for performance evaluation. PCR, polymerase chain reaction; hMPV, human metapneumovirus; ID, identification; IRB, institutional review board; RT-qPCR, reverse transcription-quantitative PCR; PPA, percent positive agreement; NPA, percent negative agreement; OPA, overall percent agreement; Ct, cycle threshold.
Ann Clin Microbiol 2025;28(4):25. Clinical evaluation of GenBody hMPV Ag Rapid Test: a diagnostic accuracy study Download image
Table 5. Sample distribution according to Ct values
Ann Clin Microbiol 2025;28(4):25. Clinical evaluation of GenBody hMPV Ag Rapid Test: a diagnostic accuracy study Download table Ct strata Ct values Ct < 24 24 ≤ Ct ≤ 28 28 < Ct Sample distribution 54.54% (30/55) 16.36% (9/55) 29.09% (16/55) Sensitivity 100% (30/30) 100% (9/9) 75% (12/16) Abbreviation: Ct, cycle threshold
Table 4. Positive sample distribution according to age
Ann Clin Microbiol 2025;28(4):25. Clinical evaluation of GenBody hMPV Ag Rapid Test: a diagnostic accuracy study Download table Age group Distribution (positive sample) Sensitivity Newborn (≤27 d) 0% (0/55) – Infant (28 d–23 m) 27.3% (15/55) 93.3% (14/15) Children (24 m–11 y) 60.0% (33/55) 97.0% (32/33) Adolescence (12 y–18 y) 3.6% (2/55) 50.0% (1/2) Adult […]
Table 3. Overall performance of the GenBody hMPV Ag Rapid Test
Ann Clin Microbiol 2025;28(4):25. Clinical evaluation of GenBody hMPV Ag Rapid Test: a diagnostic accuracy study Download table Variables Confirmatory method Total Positive Negative Positive 51 0 51 Negative 4 110 114 Total 55 110 165 Overall percent agreement (OPA) 97.58% (95% CI: 93.93%–99.05%) Percent positive agreement (PPA) 92.73% (95% CI: 82.74%–97.14%) Percent negative agreement […]
Table 2. Age distribution categories
Ann Clin Microbiol 2025;28(4):25. Clinical evaluation of GenBody hMPV Ag Rapid Test: a diagnostic accuracy study Download table Group Age Sample Count Percentage (%) Newborn Birth to 27 days 0 0 Infant 28 days to 23 months 19 11.5 Children 24 months to 11 years 75 45.5 Adolescence 12 years to 18 years 7 4.2 […]