Original article PDF Eui-Chong Kim*1,2, Jung-Hee Lee2, Hyun-Jin Jung1, and Young-Joon Lee1 Department of Clinical Pathology, Seoul National University College of Medicine1; Clinical Research Institute, Seoul National University Hospital2, Seoul, Korea Corresponding to Eui-Chong Kim, E-mail: euichong@plaza.snu.ac.kr Ann Clin Microbiol 1999;2(2):172-176.Copyright © Korean Society of Clinical Microbiology. Abstract Background: Hand-foot-mouth disease (HFMD) is mainly caused by the infection of coxsackievirus A16. But recently several epidemics of HFMD with meningitis or myocarditis due to enterovirus 71 have been reported in Southeast Asia. It was necessary that the possibility of enterovirus 71 epidemic in Korea should be ruled out. This study was designed for the determination of causative agents of HFMD in Cheju province in the spring of 1998. Methods: Serum specimens were collected from 45 pediatric patients with HFMD at Cheju Hankook Hospital in March and April, 1998. Virus isolation was performed with RD cell culture through up to three passages.

Original article PDF Eui Chong Kim, M.D.¹,², Dong Young Lee, M.D.¹, Hae Shim Choi, M.S.¹, Se Ik Joo, M.S.¹, Jung Hee Lee, M.S.³, Sang Hyun Kim, M.D.² and Sung Hwan Ban, M.D.³ ¹Department of Clinical Pathology, Seoul National University College of Medicine; ²Clinical Research Institute, Seoul National University Hospital, Seoul; ³Department of Pediatrics, Kumi Hospital, Soonchunhyang University College of Medicine, Kumi, Korea Corresponding to Eui Chong Kim, E-mail: euichong@plaza.snu.ac.kr Ann Clin Microbiol 2000;3(2):94-98.Copyright © Korean Society of Clinical Microbiology. Abstract Background: Shiga toxin-producing Escherichia coli (STEC) was found in several serotypes of E. coli including O157 serotype. Sorbitol-MacConkey agar may be useful for the detection of E. coli O157, but is not helpful for the detection of sorbitol-fermenting STEC other than O157. Moreover, some strains of E. coli O157 can ferment sorbitol. In this study, in situ hybridization using DNA probe of shiga toxin was used for the isolation of STEC from the PCR-positive stool and sequence analysis

Original article PDF Chulhun L. Chang, M.D., Dae Young Seo, M.D.,* Tae Hee Park, M.D., Jeong Seon Park, M.D., and Weon Joo Hwang, M.T. Department of Clinical Pathology, Pusan National University Hospital, Pusan; Department of Clinical Pathology,* Dong-A University Hospital, Pusan, Korea Corresponding to Chulhun L. Chang, E-mail: cchl@hyowon.cc.pusan.ac.kr Ann Clin Microbiol 2001;4(1):40-44.Copyright © Korean Society of Clinical Microbiology. Abstract Background: Mycobacterial false-positive cultures have rarely been recognized in Korea, even though the rate of false-positive cultures of Mycobaterium tuberculosis has ranged from 0.4% to 4.0%. We estimated the false-positive rates by the review of medical records from whom mycobacterial cultures were requested, retrospeaively, after a bout of false-positive cultures was discovered in specimens treated in a single day. Methods : Of the total 2,245 specimens, including 337 positive cultures of mycobacteria, during the period of January and June 1999, seventy-two specimens that showed colonies less than or equal to 5 colonies

Original article PDF Seong Geun Hong1, Sunjoo Kim2, Seok Hoon Jeong3, Chulhun L. Chang4, Sung Ran Cho5, Ji Young Ahn5, Jong Hee Shin6, Hye Soo Lee7, Won Keun Song8, Young Uh9, Jong Hwa Yum10, Dongeun Yong10, Kyungwon Lee10, Yunsop Chong10 Department of Laboratory Medicine, Coll. of Med., Pochon CHA Univ.1, Gyeongsang Nat. Univ. School of Med.2, Coll. of Med. Kosin Univ.3, Pusan Nat. Univ. Coll. of Med.4, Sooncheonhyang Univ. Coll. of Med.5, Chonnam Nat. Univ. Med. School6, Chonbuk Univ. Med. School7, Hanllym Univ. Coll. of Med.8, Yonsei Univ. Wonju Coll. of Med.9, Yonsei Univ. Coll. of Med.10 Corresponding to Kyungwon Lee, E-mail: leekcp@yumc.yonsei.ac.kr Ann Clin Microbiol 2003;6(2):149-155.Copyright © Korean Society of Clinical Microbiology. Abstract Background：Increase in extended-spectrum β-lactamase(ESBL)-producing Escherichia coli and Klebsiella pneumoniae have been reported in Korea. The aim of this study was to determine the nationwide prevalence of ESBL-producing E. coli and K. pneumoniae, and to investigate the types of ESBLs. Methods：A total of 2,221 E. coli and

Original article PDF Wee Gyo Lee1, Il Joong Park1, Ji Young Huh2, Eui-Chong Kim2, Kyungwon Lee3, Mi-Na Kim4, Chulhun L. Chang5, Sunjoo Kim6, Young Uh7, Insoo Rheem8, Gyoung Yim Ha9, Hye Soo Lee10 Department of Laboratory Medicine1, Ajou University School of Medicine, Suwon; Department of Laboratory Medicine2, Seoul National University College of Medicine; Department of Laboratory Medicine3, Yonsei University College of Medicine; Department of Laboratory Medicine4, University of Ulsan College of Medicine, and Asan Medical Center, Seoul; Department of Laboratory Medicine5, Pusan National Uinversity School of Medicine, Busan; Department of Laboratory Medicine6, Gyeongsang University College of Medicine, Jinju; Department of Laboratory Medicine7, Yonsei University Wonju College of Medicine, Wonju; Department of Laboratory Medicine8, Dankook University College of Medicine, Chunan; Department of Laboratory Medicine9, Dongguk University College of Medicine, Gyungju; and Department of Laboratory Medicine10, Chonbuk National University College of Medicine, Chonju, Korea Corresponding to Wee Gyo Lee, E-mail: weegyo@ajou.ac.kr Ann

Case report PDF Chang Ki Kim1 , Injoo Cho1 , Youn Hee Park1, Kyoung Ho Roh1, Dongeun Yong1,3,4, Kyungwon Lee1,3,4 , June Myung Kim2,3,4, Yunsop Chong1,3 Departments of Laboratory Medicine1, Internal Medicine2; Research Institute of Bacterial Resistance3; and Brain Korea 21 Project for Medical Sciences4, Yonsei University College of Medicine, Seoul, Korea Corresponding to Dongeun Yong, E-mail: deyong@yumc.yonsei.ac.kr Ann Clin Microbiol 2006;9(1):71-75.Copyright © Korean Society of Clinical Microbiology. Abstract Haemophilus aphrophilus is a facultative anaerobic, gram-negative coccobacillus or bacillus and its growth is stimulated by 5 to 10% CO2 . Most Haemophilus species require either exogenous X or V factor or both to grow, but H. aphrophilus can grow without these factors. H. aphrophilus rarely causes invasive infections such as endocarditis, septicemia, pneumonia and peritonitis in human. Two cases of infective endocarditis by H. aphrophilus have been reported in Korea. However, there has been no report of polymicrobial endocarditis by H. aphrophilus and other bacteria. We isolated H. aphrophilus and coagulase-negative staphylococci (CNS) from