Annals of Clinical Microbiology, The official Journal of the Korean Society of Clinical Microbiology

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Indexed in KCI, KoreaMed, Synapse, DOAJ
Open Access, Peer Reviewed
pISSN 2288-0585 eISSN 2288-6850

Search Results for: Jeong Hwan Shin

Evaluating the Use of Distilled Water for Washing Sodium Hydroxide in Mycobacterial Culture

Original article Hae-Gyeong Baek1, Hyun-Mi Ko2, Myung-Hee Lee1 1Department of Laboratory Medicine, Kwangju Christian Hospital, Gwangju, 2Dental Science Research Institute, Department of Oral Anatomy, School of Dentistry, Chonnam National University, Gwangju, Korea. Corresponding to Myung-Hee Lee, E-mail: purunee0820@naver.com Ann Clin Microbiol 2020;23(4):201-210. https://doi.org/10.5145/ACM.2020.23.4.5Received on 8 October 2020, Revised on 3 November 2020, Accepted on 2 December 2020, Published on 20 December 2020.Copyright © Korean Society of Clinical Microbiology. Abstract Background: Respiratory specimens subjected to mycobacterial detection were initially pre-treated with N-acetyl-L-cysteine-sodium hydroxide (NALC-NaOH) to remove the mucus and normal flora. Next, they were washed and neutralized with phosphate-buffered solution (PBS). The effectiveness of distilled water (DW) compared to PBS as a washing neutralizer during identification of mycobacteria was evaluated in this study. Methods: We analyzed the results of mycobacterial test conducted at a general hospital in Gwangju from October 2016 to September 2018. PBS and DW were used as a

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Pseudo-outbreak of Klebsiella oxytoca from Bronchial Washing Specimens

Original article PDF Ja Young Lee1, Jeong Hwan Shin1,2, Hyun-Kyung Lee3, Seong-Mi Yu5, Eun Hee Park6, Hee Ryune Lee1, Jae Hyen Kim1, Hye Ran Kim1, Chi Sook Moon3, Young Jae Kim4, Jeong Nyeo Lee1,2 1Department of Laboratory Medicine, 2Paik Institute for Clinical Research, Departments of 3Internal Medicine and 4Anesthesiology, College of Medicine, Inje University, Busan; 5Department of Nursing, Gwangju Health College, Gwangju; 6Busan Metropolitan City Institute of Health and Environment, Busan, Korea Corresponding to Jeong Hwan Shin, E-mail: jhsmile@inje.ac.kr Ann Clin Microbiol 2008;11(1):29-34.Copyright © Korean Society of Clinical Microbiology. Abstract Background: We noticed a sudden increase in the isolation of Klebsiella oxytoca from bronchial washing specimens during May to June 2006. An epidemiological investigation was conducted to identify the cause of the outbreak and to implement appropriate infection control measures.  Methods: A total of 18 isolates of K. oxytoca were found. The 14 bronchial washing specimens that yielded K. oxytoca were taken in the outpatient bronchoscopy suite, and the other 4

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Four genotypes of carbapenem-resistant Acinetobacter baumannii strains lacking OXA-23 production in Korea

Brief communication Young Ah Kim1, Seok Hoon Jeong2,3, Jong Hee Shin4, Kyeong Seob Shin5, Jeong Hwan Shin6, Young Ree Kim7, Hyun Soo Kim8, Young Uh9, Nam Hee Ryoo10 1Department of Laboratory Medicine, National Health Insurance Service Ilsan Hospital, Goyang, 2Department of Laboratory Medicine, Yonsei University College of Medicine, Seoul, 3Research Institute of Bacterial Resistance, Yonsei University College of Medicine, Seoul, 4Department of Laboratory Medicine, Chonnam National University Medical School, Gwangju, 5Department of Laboratory Medicine, Chungbuk National University College of Medicine, Cheongju, 6Department of Laboratory Medicine and Paik Institute for Clinical Research, Inje University College of Medicine, Busan, 7Department of Laboratory Medicine, Jeju National University, College of Medicine, Jeju, 8Department of Laboratory Medicine, Hallym University Dongtan Sacred Heart Hospital, Hallym University College of Medicine, Hwaseong, 9Department of Laboratory Medicine, Yonsei University Wonju College of Medicine, Wonju, 10Department of Laboratory Medicine, Keimyung University School of Medicine, Daegu, Korea Corresponding to Young Ah

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Influence of Glycopeptides, as a Risk Factor on Intestinal Colonization with Vancomycin-Resistant Enterococcus

Original article PDF Jeong Hwan Shin, M.D., Chul Hun Chang, M.D., Han Chul Son, M.D., and Kwang Ok Park*, M.D. Department of Clinical Pathology, College of Medicine, Pusan National University, and Hospital Infection Control Committee, Pusan National University Hospital* Corresponding to Jeong Hwan Shin, E-mail: jhsmile@hitel.net Ann Clin Microbiol 1998;1(1):68-74.Copyright © Korean Society of Clinical Microbiology. Abstract Background:Several risk factors related with vancomycin-resistant Enterococcus (VRE) colonization are well known, but the direct relatedness of the use of glycopeptides with VRE colonization is not confirmed yet. So we evaluated the influence of the use of glycopeptides and other variables, as risk factors on intestinal colonization with VRE. Methods:In glycopeptide-administered inpatients group, multiple stool specimens were collected on the day of glycopeptides administration, and weekly after that, until VRE were detected. In the inpatients and outpatients control groups, stool were obtained with point survey. The specimens were inoculated on m-enterococcus agar with 6mg/L vancomycin.

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Distribution and Antifungal Susceptibility of Candida species Isolated from Clinical Specimens during the Past Six Years

Original article PDF Jeong Hwan Shin, Hye Ran Kim, Jeong Nyeo Lee Department of Laboratory Medicine, Busan Paik Hospital, College of Medicine, Inje University, Busan, Korea Corresponding to Jeong Hwan Shin, E-mail: jhsmile@inje.ac.kr Ann Clin Microbiol 2004;7(2):164-170.Copyright © Korean Society of Clinical Microbiology. Abstract Background : The epidemiology of Candida species isolated from nonsterile as well as normally sterile sites is important because colonization of the former may precede invasive Candida infections. Methods : We investigated the epidemiology and antifungal susceptibility of Candida species recovered in Busan Paik Hospital during the past 6 years and compared these results according to the type of specimens. Results : Among the 2364 strains, C. albicans(53.8%) was the most frequently isolated, followed by C. tropicalis (17.5%), and C. guilliermondii (10.0%). Non-albicans Candida species were more prevalent in normally sterile sites (P<0.001); the prevalence of C. tropicalis and C. parapsilosis was significantly higher in normally sterile than in nonsterile sites (P<0.001). The prevalence of C. parapsilosis was higher in blood, intravenous catheter tips,

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Etiology and Antimicrobial Susceptibility of Bacterial Pathogens Causing Community-Acquired Urinary Tract Infection at a Tertiary-care Hospital

Original article PDF Jeong Hwan Shin1,5, Hye Ran Kim1, Hi Ryune Lee1, Jae Il Chung2, Kweonsik Min2,5, Chi Sook Moon3, Seong Mi Ryu4, and Jeong Nyeo Lee1,5 Departments of Laboratory Medicine1, Urology2, and Internal Medicine3, College of Medicine, Paik Institute for Clinical Research5, Inje University, Infection Control Committee4, Inje University Busan Paik Hospital, Busan, Korea Corresponding to Jeong Hwan Shin, E-mail: jhsmile@inje.ac.kr Ann Clin Microbiol 2005;8(2):142-147.Copyright © Korean Society of Clinical Microbiology. Abstract Background: Resistant organisms are now a growing and frequent problem in community-acquired infections. There is little information on the etiology and antimicrobial susceptibility patterns of community-acquired urinary tract infection (CA-UTI) at a tertiary-care hospital. Methods: We evaluated the distribution of etiological organisms with their antimicrobial susceptibility patterns of CA-UTI in the patients visiting a tertiary-care hospital during the period of three years from 2001 through 2003. Results: In total, 1,753 bacterial isolates yielded a significant growth as

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Evaluation of a Colorimetric Antifungal Susceptibility Test by 2,3-Diphenyl-5-Thienyl-(2)-Tetrazolium Chloride for Fluconazole in Candida Species Isolated from Clinical Specimens

Original article PDF Jae Hyen Kim1, Jeong Hwan Shin1,2, Eun Jung Lee1,2, Ja Young Lee1, Hye Ran Kim1, Chulhun Ludgerus Chang3, Jeong Nyeo Lee1,2 1Department of Laboratory Medicine and 2Paik Institute for Clinical Research, College of Medicine, Inje University; 3Department of Laboratory Medicine, School of Medicine, Pusan National University, Busan, Korea Corresponding to Jeong Hwan Shin, E-mail: jhsmile@inje.ac.kr Ann Clin Microbiol 2007;10(2):90-95.Copyright © Korean Society of Clinical Microbiology. Abstract Background: The aims of this study were to evaluate the colorimetric antifungal susceptibility test to fluconazole using 2,3-diphenyl-5-thienyl-(2)-tetrazolium chloride (STC) for various Candida species isolated from clinical specimens and to compare the results with those of the CLSI M27-A2 standard method. Methods: The fluconazole MICs for 204 clinical Candida isolates consisting of 100 C. albicans, 45 C. glabrata, 28 C. tropicalis, 22 C. parapsilosis, and 9 other Candida species were determined by the CLSI and STC colorimetric methods. Results: All 204 Candida strains were grown on the growth control wells of CLSI standard plates, but

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Septic Peripheral Embolism in Left Leg from Aggregatibacter aphrophilus Endocarditis

Case report PDF Ja Young Lee1, Si Hyun Kim1, Haeng Soon Jeong1, Seung Hwan Oh1, Hye Ran Kim1, Young Il Yang2,4, Yang Haeng Lee3, Jeong Nyeo Lee1,4, Jeong Hwan Shin1,4 Departments of 1Laboratory Medicine, 2Pathology, 3Thoracic Surgery, 4Paik Institute for Clinical Research, Inje University College of Medicine, Busan, Korea Corresponding to Jeong Hwan Shin, E-mail: jhsmile@inje.ac.kr Ann Clin Microbiol 2009;12(2):82-86.Copyright © Korean Society of Clinical Microbiology. Abstract Aggregatibacter aphrophilus is a facultatively anaerobic gram-negative coccobacillus or bacillus that grows with no dependence on X factor and variable requirement for V factor. The organism is normal flora in the human oral cavity and upper respiratory tract and, rarely, causes invasive infections such as bacteremia, endocarditis, brain abscess, or osteomyelitis. We report a case of septic peripheral embolism in left leg from A. aphrophilus endocarditis. A 49-year-old man with known hypertension presented with acute muscle pain in the left leg. On physical examination, a regular heartbeat with a pansystolic

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Antimicrobial resistance patterns of Acinetobacter baumannii and Pseudomonas aeruginosa isolated from vulnerable patients in Korea, 2021

Original article Young Ah Kim1, Seok Hoon Jeong2,3, Jong Hee Shin4, Kyeong Seob Shin5, Jeong Hwan Shin6, Young Ree Kim7, Hyun Soo Kim8, Young Uh9, Nam Hee Ryoo10 1Department of Laboratory Medicine, National Health Insurance Service Ilsan Hospital, Goyang, 2 Department of Laboratory Medicine, Yonsei University College of Medicine, Seoul, 3Research Institute of Bacterial Resistance, Yonsei University College of Medicine, Seoul, 4Department of Laboratory Medicine, Chonnam National University Medical School, Gwangju, 5Department of Laboratory Medicine, Chungbuk National University College of Medicine, Cheongju, 6Department of Laboratory Medicine and Paik Institute for Clinical Research, Inje University College of Medicine, Busan, 7Department of Laboratory Medicine, Jeju National University College of Medicine, Jeju, 8Department of Laboratory Medicine, Hallym University Dongtan Sacred Heart Hospital, Hallym University College of Medicine, Hwaseong, 9Department of Laboratory Medicine, Yonsei University Wonju College of Medicine, Wonju, 10Department of Laboratory Medicine, Keimyung University School of Medicine, Daegu, Korea Corresponding to Young

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Current prevalence of the crpP gene in carbapenemase-producing Pseudomonas aeruginosa blood isolates in Korea

Original article Jinho Heo1, Yu Jeong Choi1, Young Ah Kim2, Seok Hoon Jeong1,3, Jong Hee Shin4, Kyeong Seob Shin5, Jeong Hwan Shin6, Young Ree Kim7, Hyun Soo Kim8, Young Uh9, Nam Hee Ryoo10 1Department of Laboratory Medicine, Yonsei University College of Medicine, Seoul, 2Department of Laboratory Medicine, National Health Insurance Service Ilsan Hospital, Goyang, 3Research Institute of Bacterial Resistance, Yonsei University College of Medicine, Seoul, 4Department of Laboratory Medicine, Chonnam National University Medical School, Gwangju, 5Department of Laboratory Medicine, Chungbuk National University College of Medicine, Cheongju, 6Department of Laboratory Medicine and Paik Institute for Clinical Research, Inje University College of Medicine, Busan, 7Department of Laboratory Medicine, Jeju National University, College of Medicine, Jeju, 8Department of Laboratory Medicine, Hallym University Dongtan Sacred Heart Hospital, Hallym University College of Medicine, Hwaseong, 9Department of Laboratory Medicine, Yonsei University Wonju College of Medicine, Wonju, 10Department of Laboratory Medicine, Keimyung University School of Medicine, Daegu,

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