Fig. 2. Morphological analysis of Acanthamoeba spp. using calcofluor-white staining. (A) Acanthamoeba cysts in a group under light microscopy (400×, scale bar = 10 μm). (B) Acanthamoeba trophozoites with a single large nucleus and numerous vacuoles were conspicuous under light microscopy (200×, scale bar = 10 μm). (C) Remarkable Acanthamoeba cysts with double cyst walls under fluorescence microscopy (400×, scale bar = 10 μm). (D) Acanthamoeba trophozoites were relatively unremarkable under fluorescence microscopy (400×, scale bar = 10 μm).
Ann Clin Microbiol 2024;27:149-153. Can Acanthamoeba keratitis be properly diagnosed without culture in the real-world clinical microbiology laboratory?: a case report
Fig. 1. Anterior segment photographs of the patient in this case study. The top two anterior segment images were taken under slit-lamp microscope (A, B) and the bottom two images were under cobalt blue light after fluorescein dye staining (C, D). During the slit-lamp microscopy examination of the patient’s left eye, radial keratoneuritis was observed in the central corneal stroma, while vitreous and retina showed no abnormalities.
Ann Clin Microbiol 2024;27:149-153. Can Acanthamoeba keratitis be properly diagnosed without culture in the real-world clinical microbiology laboratory?: a case report
Fig. 2. The clinical course of dengue shows the three distinct phases (illustration reproduced from Yacoub et al. [23], with permission).
Ann Clin Microbiol 2024;27:131-141. Dengue fever: epidemiology, clinical manifestations, diagnosis, and therapeutic strategies
Fig. 1. Dengue virus transmission cycle (illustration adapted from Gómez et al. [28]).
Ann Clin Microbiol 2024;27:131-141. Dengue fever: epidemiology, clinical manifestations, diagnosis, and therapeutic strategies
Fig. 4. Species of anisakid larvae detected in humans (nationwide, mostly from Seoul) and fish/ squids from the eastern, southern, and western seas of Korea based on molecular analyses [3,107,113,178,180,181,184-189,205-213]. Av, anchovy; Cm, chub mackerel; Cs, chub salmon; Jf, Japanese flounder; Gl, greenling; H, human; Ht, hairtail; Lh, large-head hairtail; Ms, masou salmon; Pc, Pacific cod; Rf, rockfish; Se, sea eel; Sq, squid; Vf, various fish; Wp, walleye pollock.
Ann Clin Microbiol 2024;27:93-130. Anisakidosis in humans and animals and detection of anisakid larvae in fish and cephalopods in Korea: a literature review (1971-2022)
Fig. 3. Involved organs in 851 anisakidosis cases reported in Korea (1971-2022).
Ann Clin Microbiol 2024;27:93-130. Anisakidosis in humans and animals and detection of anisakid larvae in fish and cephalopods in Korea: a literature review (1971-2022)
Fig. 2. Number of anisakidosis cases according to localities in Korea (1971-2022).
Ann Clin Microbiol 2024;27:93-130. Anisakidosis in humans and animals and detection of anisakid larvae in fish and cephalopods in Korea: a literature review (1971-2022)
Fig. 1. Number of anisakidosis cases reported in scientific articles in Korea (1971-2022).
Ann Clin Microbiol 2024;27:93-130. Anisakidosis in humans and animals and detection of anisakid larvae in fish and cephalopods in Korea: a literature review (1971-2022)
Fig. 1. Functionality of Trichomonas vaginalis actin LAMP assays. (A) LAMP on 10-fold serial dilutions of T. vaginalis genomic DNA (10 ng to 1 pg per reaction) monitored by measuring absorbance. Distilled water was used as a negative control. (B) LAMP products were visualized by gel electrophoresis. Lane 1, 1 ng; Lane 2, 100 pg; Lane 3, 10 pg; Lane 4, 1 pg of T. vaginalis genomic DNA; Lane 5, LAMP product after HindIII digestion; Lane 6, distilled water; andLane M, 100-bp DNA marker. (C) LAMP products were visualized under UV light using Loopamp fluorescent detection reagent. Lane 1, 1 ng; Lane 2, 100 pg; Lane 3, 10 pg; Lane 4, 1 pg; Lane 5, 100 fg; Lane 6, 10 fg of T. vaginalis genomic DNA; and Lane 7, distilled water. (D and E) T. vaginalis at a density of 1×102 parasites/μL was serially diluted and tested using LAMP assay (D) and PCR (E) with F3 and B3 primers (Table 1). Lane M, 100-bp DNA marker; Lane 1, 100; Lane 2, 10; Lane 3, 1; Lane 4, 0.1; Lane 5, 0.01 parasite (s) per reaction; Lane 6, positive control, 100 pg of plasmid DNA containing LAMP targeting regions of actin gene; and Lane 7, distilled water. (F) Specificity of LAMP primers for detection of T. vaginalis assessed using template DNA from other microbial species. Lane 1, T. vaginalis; Lane 2, Candida albicans; Lane 3, Chlamydia trachomatis; Lane 4, Neisseria gonorrhoeae; Lane 5, Cryptosporidium parvum; Lane 6, Entamoeba histolytica; Lane 7, Giardia lamblia; Lane 8, Escherichia coli; and Lane 9, human genomic DNA. LAMP products were visualized via color change that was also observable by the naked eye under normal visible light reproduced under the CC-BY-NC license from the original article [19].
Ann Clin Microbiol 2024;27:69-79. Molecular diagnosis of parasitic diseases in Korea
Fig. 5. The nematode eggs (Group IV). A. Ascaris lumbricoides (F); B. A. lumbricoides (F) in CTS specimen; C. A. lumbricoides (U); D. A. lumbricoides (U) in CTS specimen; E. Trichuris trichiura; F. T. trichiura in CTS specimen; G. Enterobius vermicularis in CTS specimen; H. E. vermicularis in a specimen of cellotape anal swab; I. Hookworm; J. Hookworm (morular stage) in CTS specimen; K. Air-bubble like hookworm egg of which a mature larva already escaped; L. Trichostorongylus orientalis. Scale bar = 25 ㎛. CTS, cellophane thick smear.
Ann Clin Microbiol 2024;27:49-67. Practical guide for the diagnosis of helminth ova in stools