Case report PDF Kyong-Ah Yun, M.D., Mi-Na Kim, M.D., Chik Hyun Pai, M.D., and Han Joo Lee*, M.D. Departments of Clinical Pathology and Internal Medicine*, University of Ulsan, College of Medicine and Asan Medical Center, Seoul Corresponding to Mi-Na Kim, E-mail: mnkim@amc.seoul.kr Ann Clin Microbiol 2001;4(1):68-71.Copyright © Korean Society of Clinical Microbiology. Abstract Yersinia pseudotuberculosis is a relatively infrequent cause of human infections, mostly as intestinal yersinosis. A septicemic form of Y. pseudotuberculosis infection has been reported only rarely. It is usually seen in patients with underlying disorders such as diabetes, hepatic cirrhosis or iron overload. A 63-year-old man with diabetes mellitus and liver fibrosis was admitted to Asan Medical Center via emergency department because of epigastric pain, fever and watery diarrhea; he was septic. The stool culture did not grow Salmonella, Shigella, or Yersinia. But, in the blood culture Y. pseudotuberculosis grew from one anaerobic vial among two sets of aerobic and anaerobic blood cultures. Serotype of Y.

Original article PDF Young-Uk Cho, Jeong-Don, Hye-Young Park, Mi-Na Kim Department of Laboratory Medicine and Research Institute of Bacterial Resistance, Yonsei University College of Medicine, Seoul, Korea Corresponding to Mi-Na Kim, E-mail: mnkim@amc.seoul.kr Ann Clin Microbiol 2002;5(2):111-118.Copyright © Korean Society of Clinical Microbiology. Abstract Background:Coagulase-negative staphylococci (CNS) has been considered as a major causative agent of nosocomial infections. A prompt and accurate detection of methicillin resistance (MR) in staphylococci is a current issue of clinical microbiology laboratories. This study was purposed to evaluate various methods for detecting MR from CNS. Methods:We selected 78 CNS strains obtained from blood cultures from April 1999 through July 2001 including 20 strains of Staphylococcus epidermidis, 20 S. hominis (SHO), 19 S. capitis, 9 S. haemolyticus, 3 S. saccharolyticus, 1 S. saprophyticus (SAP), 2 S. warneri (SWA), 2 S. xylosus, 1 S. lugdunensis, and 1 S. auricularis. In addition, one SAP strain received from World Health Organization for proficiency tests was also studied. The following methods were compared to the mecA gene PCR: MicroScan

Case report PDF Choong Hwan Cha, Hee Bong Shin, Seongsoo Jang, Min Kyung Kim*, Yang Soo Kim*, Jae Kawn Song* and Mi-Na Kim Department of Laboratory Medicine and Internal Medicine*, University of Ulsan College of Medicine and Asan Medical Center, Seoul, Korea Corresponding to Mi-Na Kim, E-mail: mnkim@amc.seoul.kr Ann Clin Microbiol 2003;6(2):172-176.Copyright © Korean Society of Clinical Microbiology. Abstract Haemophilus aphrophilus is a facultatively anaerobic gram-negative bacillus and require 5 to 10 % to grow optimally. H. aphrophilus is differentiated from other members of Haemophilus species by no requirement of X or V factor. This organism is found as the normal flora in upper respiratory tract but a member of the HACEK group that cause native valve endocarditis. Since the first endocarditis of H. aphrophilus was reported at 1985 in Korea, we reported the second case.  A 35-year-old male patient was admitted to Asan Medical Center because of fever for 15 days and altered mentality developed 2

Original article PDF Seong Geun Hong1, Dongeun Yong2, Kyungwon Lee2, Eui-Chong Kim3, Wee Kyo Lee4, Seok Hoon Jeong5, Won Keun Song6, Yeon Jun Park7, Mi-Na Kim8, Young Uh9, Jong Hee Shin10, Jongwook Lee11, Ji Young Ahn12, Sun Wha Lee13, Jae Seok Kim5, Hee Bong Shin1 Departments of Laboratory Medicine, Coll. of Med., Pochon CHA Univ.1, Yonsei Univ. Coll. of Med.2 Seoul Nat. Univ. Coll. of Med.3, Ajou Univ. of Med.4, Coll. of Med. Kosin Univ.5, Hanllym Univ. Coll. of Med.6 The Catholic Univ. of Kor.7, Univ. of Ulsan Med. Coll. and Asan Med. Center8, Yonsei Univ. Wonju Coll. of Med.9 Chonnam Nat. Univ. Med. School10, Keonyang Univ. Med. Coll.11 Sooncheonhyang Univ. Coll. of Med.12; Neodin Med. Res. Center13 Corresponding to Kyungwon Lee, E-mail: leekcp@yumc.yonsei.ac.kr Ann Clin Microbiol 2003;6(1):29-36.Copyright © Korean Society of Clinical Microbiology. Abstract Background: A rapid increase of antimicrobial-resistant bacteria has become a serious problem in many countries. The aim of this

Original article PDF Hye Gyung Bae1, Yong-Hak Sohn1, Jong Hee Shin2, Mi-Na Kim1 Department of Laboratory Medicine1, University of Ulsan College of Medicine and Asan Medical Center, Seoul, Korea; Department of Laboratory Medicine2, Chonnam National University Medical School, Gwangju, Korea Corresponding to Mi-Na Kim, E-mail: mnkim@www.amc.seoul.kr Ann Clin Microbiol 2004;7(2):156-163.Copyright © Korean Society of Clinical Microbiology. Abstract Background : Although the National Committee for Clinical Laboratory Standards (NCCLS) defined a standard reference broth microdilution method for testing the susceptibility of Candida species to antifungal drugs, many clinical laboratories require easier but reliable alternatives for routine antifungal susceptibility testing. We evaluated ATB FUNGUS 2 (bioMerieux, France.; ATB) compared to the method recommended by the NCCLS (NCCLS). Methods : A total of 28 strains of Candidaspecies consecutively isolated from blood and CSF cultures at Asan Medical Center from April to June 2004 were tested. In addition, 12 strains comprising C. krusei (3), C. glabrata (7) and C.

Original article PDF Heungsup Sung, Hee Jung Chung, Yeon Jung Pyo, Seung Namgoong, and Mi-Na Kim* Department of Laboratory Medicine, University of Ulsan College of Medicine and Asan Medical Center, Seoul, Korea Corresponding to Mi-Na Kim, E-mail: mnkim@amc.seoul.kr Ann Clin Microbiol 2005;8(2):113-120.Copyright © Korean Society of Clinical Microbiology. Abstract Background: Because the mortality rate of invasive aspergillosis (IA) is more than 50%, an early diagnosis and appropriate management are important to achieve a favorable outcome. Aspergillus galactomannan (AG) antigen test has recently been introduced for diagnosis and monitoring of IA. This study was to evaluate the clinical utility of AG detection in diagnosis of IA. Methods: One hundred and seventy-five samples from 149 patients were tested for AG during the period from September 2004 to May 2005 and the results were evaluated retrospectively. IA was diagnosed into ‘proven’, ‘probable’and ‘possible’,groups based on patients’clinical laboratory findings as per European Organization for Research and

Original article PDF Wee Gyo Lee1, Il Joong Park1, Ji Young Huh2, Eui-Chong Kim2, Kyungwon Lee3, Mi-Na Kim4, Chulhun L. Chang5, Sunjoo Kim6, Young Uh7, Insoo Rheem8, Gyoung Yim Ha9, Hye Soo Lee10 Department of Laboratory Medicine1, Ajou University School of Medicine, Suwon; Department of Laboratory Medicine2, Seoul National University College of Medicine; Department of Laboratory Medicine3, Yonsei University College of Medicine; Department of Laboratory Medicine4, University of Ulsan College of Medicine, and Asan Medical Center, Seoul; Department of Laboratory Medicine5, Pusan National Uinversity School of Medicine, Busan; Department of Laboratory Medicine6, Gyeongsang University College of Medicine, Jinju; Department of Laboratory Medicine7, Yonsei University Wonju College of Medicine, Wonju; Department of Laboratory Medicine8, Dankook University College of Medicine, Chunan; Department of Laboratory Medicine9, Dongguk University College of Medicine, Gyungju; and Department of Laboratory Medicine10, Chonbuk National University College of Medicine, Chonju, Korea Corresponding to Wee Gyo Lee, E-mail: weegyo@ajou.ac.kr Ann

Original article PDF Kyung Ran Jun, Hong Seon Jeon, Heungsup Sung, Mi-Na Kim Department of Laboratory Medicine, University of Ulsan College of Medicine and Asan Medical Center, Seoul, Korea Corresponding to Mi-Na Kim, E-mail: mnkim@amc.seoul.kr Ann Clin Microbiol 2006;9(1):58-63.Copyright © Korean Society of Clinical Microbiology. Abstract Background: We evaluated the BD Phoenix Automated Microbiology System (Phoenix) for its ability to detect methicillin resistant Staphylococcus aureus(MRSA) and compared the results to those obtained by the Clinical and Laboratory Standards Institute (CLSI) agar dilution method, a mecA gene PCR method, and the MicroScan WalkAway 96 System (MicroScan). Methods: One hundred seventy S. aureus strains (Group I) isolated from blood and urine cultures were collected from eight university hospitals and 58 strains (Group II) including 20 blood isolates among Group I and 38 isolates from skin lesions of atopic patients were collected from Asan Medical Center. All 208 isolates were tested with Phoenix using PMIC/ID-53 panels, and the

Original article PDF Hee Young Chung, M.D.1, Yoo Li Kim, Ph.D.2, Kyung Ah Hwang, Ph.D.2, Byung Hoo Choi, M.T.1, Sook Ja Park, M.T.1, Heung Sup Sung, M.D.1, Mi-Na Kim, M.D.1 1Department of Laboratory Medicine, University of Ulsan College of Medicine, Asan Medical Center, 2 BioSewoom Institutue Bioscience and Biotechnology, Seoul, Korea Corresponding to Mi-Na Kim, E-mail: mnkim@amc.seoul.kr Ann Clin Microbiol 2007;10(2):77-83.Copyright © Korean Society of Clinical Microbiology. Abstract Background: We evaluated the performance of a newly developed real-time polymerase chain reaction (PCR) method using TaqMan probe (TP) and internal control (IC) for quantitation of BK virus (BKV) DNA. Methods: PCR primers and TP were targeted for the VP1 of BKV and 300 bp-region of VP1 was cloned to prepare a standard DNA. Threshold cycles (Ct) of IC was set at 33±3. The recovery rates, precision, linearity, and limit of detection (LOD) were measured using the standard DNA. To correlate TP with previous

Case report PDF Kyung Ran Jun1, Mi-Sook Jang1, Sook Ja Park1, Mi-Na Kim1, Dong Yoon Kim2, Hungwon Tchah2, Myoung Joon Kim2 Departments of 1Laboratory Medicine and 2Ophthalmology, University of Ulsan College of Medicine and Asan Medical Center, Seoul, Korea Corresponding to Mi-Na Kim, E-mail: mnkim@amc.seoul.kr Ann Clin Microbiol 2007;10(1):73-76.Copyright © Korean Society of Clinical Microbiology. Abstract Beauveria bassiana is a hyaline Hypomycetes, which is known as an insect pathogen causing infections in silkworm. It is a rare opportunistic pathogen of human accounted for pulmonary infection, keratitis, and deep tissue infection. We report the first case of B. bassiana keratitis in Korea. A 64-year-old man with a 10-year history of herpetic keratitis was referred for the treatment of infectious keratitis in the right eye. Corneal scrapings showed septate hyaline hyphae on calcoflour white-KOH preparation and their cultures grew B. bassiana. At the beginning, the patient was treated empirically with an antiviral and antibiotics, and then the treatment was changed with