Table 3. Summary of discrepancies in 13 strains showing discrepant results between modified-EUCAST rapid direct and conventional ASTs
Ann Clin Microbiol 2020;23:125-134. Performance of Modified-EUCAST Rapid Direct Antimicrobial Susceptibility Testing on Clinical Urine Samples Download table Species Antimicrobial agent Modified-EUCAST rapid direct AST Conventional AST MIC by E-test (μg/ml) Interpretation Escherichia coli Aztreonam R S 0.5 ME Escherichia coli Cefepime R S 4 MnE Escherichia coli Cefepime R S 8 MnE Escherichia coli […]
Table 2. Performance of modified-EUCAST rapid direct AST compared with conventional AST
Ann Clin Microbiol 2020;23:125-134. Performance of Modified-EUCAST Rapid Direct Antimicrobial Susceptibility Testing on Clinical Urine Samples Download table Antimicrobial agents Total CA MnE ME VME No. (%) No. (%) No. (%) No. (%) Ampicillin 103 99 (96.1) 3 (2.9) 0 (0) 1 (1.0) Piperacillin-tazobactam 103 94 […]
Table 1. AST performance of modified-EUCAST rapid direct and conventional AST methods
Ann Clin Microbiol 2020;23:125-134. Performance of Modified-EUCAST Rapid Direct Antimicrobial Susceptibility Testing on Clinical Urine Samples Download table Antimicrobial agents Modified-EUCAST rapid direct AST Conventional AST Unweighted kappa (95% CI) Weighted kappa (95% CI) S I R S I R Ampicillin 12 3 88 12 0 91 0.83 (0.77 – […]
Fig. 1. Flowchart of urine samples selection
Ann Clin Microbiol 2020;23:125-134. Performance of Modified-EUCAST Rapid Direct Antimicrobial Susceptibility Testing on Clinical Urine Samples Download image
Table 1. Clinical performance of STANDARD F Strep A Ag FIA and Sofia Strep A FIA compared to throat culture (n=372)
Ann Clin Microbiol 2020;23:117-124. Performance Evaluation of STANDARD F Strep A Ag FIA for Diagnosis of Group A Streptococcal Pharyngitis Download table Rapid antigen detection tests Results Throat culture Sensitivity (95% CI) Specificity (95% CI) GAS Positive GAS Negative STANDARD F Strep A Ag FIA Positive 96 13 95.0% (88.8-98. 4%) 95.2% (91.9-97. […]
Fig. 1. Distribution of age and sex of STANDARD F Strep A Ag FIA positive patients
Ann Clin Microbiol 2020;23:117-124. Performance Evaluation of STANDARD F Strep A Ag FIA for Diagnosis of Group A Streptococcal Pharyngitis Download image
Fig. 2. Relationship between adeJ pump gene expression and resistance to each antibiotics. Relative expression of adeJ efflux pump gene in non-susceptible (NS) group was higher than that of susceptible (S) group to each antibiotics in general. The statistically significant difference was found between S and NS groups to cefepime, cefotaxime, ceftazidime, ciprofloxacin, gentamicin, imipenem, meropenem, piperacillin, piperacillin/tazobactam, ticarcillin/clavulanic acid, tigecycline, and trimethoprim/sulfamethoxazole, respectively. *P < 0.05, **P < 0.001. The error bars represent the standard error of the mean.
Ann Clin Microbiol 2020;23:45-54. Role of Efflux Pump Gene adeIJK to Multidrug Resistance in Acinetobacter baumannii Clinical Isolates Download image
Fig. 1. Relative expression of the adeJ efflux pump gene determined by real-time reverse transcriptase PCR in A. baumannii type strain (ATCC19606), multidrug resistant A. baumannii AYE strain, 70 strains of multidrug resistant A. baumannii (MDRAB) and 30 strains of non-MDRAB clinical isolates. The normalized expression of adeJ was calibrated with the expression of A. baumannii ATCC 19606. The error bars represent the standard error of the mean. *P < 0.05.
Ann Clin Microbiol 2020;23:45-54. Role of Efflux Pump Gene adeIJK to Multidrug Resistance in Acinetobacter baumannii Clinical Isolates Download image
Table 1. Oligonucleotide primers used in this study
Ann Clin Microbiol 2020;23:45-54. Role of Efflux Pump Gene adeIJK to Multidrug Resistance in Acinetobacter baumannii Clinical Isolates Download table PCR Gene Primer Sequences (5′ → 3′) AT References Conventional PCR gyrB Multiplex 1 Sp4F CAC GCC GTA AGA GTG CAT TA 60°C (11) Sp4R AAC GGA GCT TGT CAG GGT TA […]
Fig. 3. Correlation between bloodstream infection (BSI) by glucose non-fermenters and average monthly temperature from 2008–2016. (a) Temperature (°C) vs. incidence rate of community-onset BSI by Acinetobacter spp. (cases per 105 patient days), y = 0.0500x+2.065, r = 0.2100, P = 0.0292. (b) Temperature (°C) vs. incidence rate of hospital-acquired BSI by Acinetobacter spp. (cases per 106 patient days), y = -0.121x+25.202, r = -0.1148, P = 0.2368. (c) Temperature (°C) vs. incidence rate of community-onset BSI by P. aeruginosa (cases per 105 patient days), y = 0.000520x+0.455, r = 0.0137, P = 0.8879. (d) Temperature (°C) vs. incidence rate of hospital-acquired BSI by P. aeruginosa (cases per 106 patient days), y = 0.0565x+8.814, r = -0.0957, P = 0.3241. CO, community-onset; HA, hospital-acquired; Temp., temperature.
Ann Clin Microbiol 2020;23:33-43. Season and Temperature Effects on Bloodstream Infection Incidence in a Korean Tertiary Referral Hospital Download image (a) Community-onset BSI by Acinetobacter spp. (b) Hospital-acquired BSI by Acinetobacter spp. (c) Community-onset BSI by P. aeruginosa (d) Hospital-acquired BSI by P. aeruginosa