Fig. 1. Distribution of blood volume in blood culture bottles. The optimal volume (8–12 mL) accounted for (A) 9.1% in preinterventional period and (B) 37.8% in post-interventional period.
Ann Clin Microbiol 2021;24:1-9. Educational Intervention to Improve Blood Culture Indicators in a Secondary-Care Hospital Download image
Table 6. Concordance of AdvanSure plus PCR, AdvanSure PCR assay for detection of MTC and NTM
Ann Clin Microbiol 2020;23:105-116. Evaluation of the AdvanSure TB/NTM Plus Real-Time PCR Assay for the Simultaneous Detection of Mycobacterium tuberculosis and Nontuberculous Mycobacteria from Clinical Specimens Download table AdvanSure PCR MTC MTC and NTM Negative NTM Total No. (%) AdvanSure Plus PCR MTC 67 0 0 0 67 (33.7) MTC and NTM 3 0 0 […]
Table 5. The performance of AdvanSure plus PCR, AdvanSure PCR assay, and AFB stain for detection of MTC/NTM compared with mycobacterial culture
Ann Clin Microbiol 2020;23:105-116. Evaluation of the AdvanSure TB/NTM Plus Real-Time PCR Assay for the Simultaneous Detection of Mycobacterium tuberculosis and Nontuberculous Mycobacteria from Clinical Specimens Download table Sensitivity % (No.) Specificity % (No.) PPV% NPV% AdvanSure PCR MTC 100.0 (45/45) 83.8 (129/154*) 32.1 100.0 NTM 64.0 (16/25) 95.4 (166/174‡) AdvanSure […]
Table 4. Results of AdvanSure plus PCR, AdvanSure PCR assay and AFB staining according to the results of culture
Ann Clin Microbiol 2020;23:105-116. Evaluation of the AdvanSure TB/NTM Plus Real-Time PCR Assay for the Simultaneous Detection of Mycobacterium tuberculosis and Nontuberculous Mycobacteria from Clinical Specimens Download table Culture MTC (n=45) No. (%) Negative (n=129) No. (%) NTM (n=25) No. (%) AdvanSure PCR MTC 45 (100) 25 (19.4) 0 Negative 0 […]
Table 3. Results of AdvanSure plus PCR and AdvanSure PCR in 7 cases of MTC and NTM mix
Ann Clin Microbiol 2020;23:105-116. Evaluation of the AdvanSure TB/NTM Plus Real-Time PCR Assay for the Simultaneous Detection of Mycobacterium tuberculosis and Nontuberculous Mycobacteria from Clinical Specimens Download table Species AdvanSure Plus PCR AdvanSure PCR MTC Ct NTM Ct IC Ct Result MTC Ct NTM Ct IC Ct Result M. intracellulare 33.57 17.87 24.06 MTC and […]
Table 2. Performance of AdvanSure plus PCR in 200 mycobacterial strains.
Ann Clin Microbiol 2020;23:105-116. Evaluation of the AdvanSure TB/NTM Plus Real-Time PCR Assay for the Simultaneous Detection of Mycobacterium tuberculosis and Nontuberculous Mycobacteria from Clinical Specimens Download table Culture AdvanSure Plus PCR MTC (n=97) MTC and NTM (n=7) NTM(n=96) MTC 97 3 0 NTM 0 4 96 M. abscessus 0 0 14 […]
Table 1. Frequency of Mycobacterium species in this study
Ann Clin Microbiol 2020;23:105-116. Evaluation of the AdvanSure TB/NTM Plus Real-Time PCR Assay for the Simultaneous Detection of Mycobacterium tuberculosis and Nontuberculous Mycobacteria from Clinical Specimens Download table Species No. % MTC 100 50.0 NTM 100 50.0 M. abscessus 14 6.2 M. avium species 28 12.4 M. kansasii 4 […]
Table 6. Diagnostic accuracy of three real-time PCR assays for NTM detection stratified by AFB smear results (n=261)
Ann Clin Microbiol 2020;23:93-104. Evaluation of Diagnostic Performance of Three Real-Time PCR Assays for the Detection of Mycobacteria Species Download table Parameter AFB smear AdvanSure Genedia PowerChek Result 95% CI Result 95% CI Result 95% CI Sensitivity (%) Positive 90.9 70.8-98.9 54.6 32.2-75.6 95.5 77.2-99.9 Negative 40.0 5.3-85.3 0.0 0.0-52.2 60.0 14.7-94.7 Total 81.5 61.9-93.7 […]
Table 5. Diagnostic accuracy of three real-time PCR assays for the MTB detection stratified by AFB smear results (n=261)
Ann Clin Microbiol 2020;23:93-104. Evaluation of Diagnostic Performance of Three Real-Time PCR Assays for the Detection of Mycobacteria Species Download table Parameter AFB smear AdvanSure Genedia PowerChek Result 95% CI Result 95% CI Result 95% CI Sensitivity (%) Positive 98.4 91.5-100.0 96.8 89.0-99.6 98.4 91.5-100.0 Negative 0.0 0.0-79.3 0.0 0.0-79.3 0.0 0.0-79.3 Total 96.9 89.2-99.6 […]
Table 4. Agreement between AFB culture tests and three real-time PCR assays for MTB and NTM detection (n=261)
Ann Clin Microbiol 2020;23:93-104. Evaluation of Diagnostic Performance of Three Real-Time PCR Assays for the Detection of Mycobacteria Species Download table Parameter Target organism AdvanSure Genedia PowerChek Result 95% CI Result 95% CI Result 95% CI PPA (%) MTB 96.9 89.3-99.1 95.3 87.1-98.4 96.9 89.3-99.1 NTM 81.8 65.6-91.4 44.4 27.6-62.7 88.9 71.9-96.1 PNA (%) MTB […]